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Mediator subunits MED16, MED14, and MED2 are required for activation of ABRE-dependent transcription in Arabidopsis

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Lee, Morgan, Dominguez-Ferreras, Anna, Kaliyadasa, Ewon, Huang, Wei-Jie, Antony, Edna, Stevenson, Tracey, Lehmann, Silke, Schäfer, Patrick, Knight, Marc R., Ntoukakis, Vardis and Knight, Heather (2021) Mediator subunits MED16, MED14, and MED2 are required for activation of ABRE-dependent transcription in Arabidopsis. Frontiers in Plant Science, 12 . 649720. doi:10.3389/fpls.2021.649720

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Official URL: http://dx.doi.org/10.3389/fpls.2021.649720

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Abstract

The Mediator complex controls transcription of most eukaryotic genes with individual subunits required for the control of particular gene regulons in response to various perturbations. In this study, we reveal the roles of the plant Mediator subunits MED16, MED14, and MED2 in regulating transcription in response to the phytohormone abscisic acid (ABA) and we determine which cis elements are under their control. Using synthetic promoter reporters we established an effective system for testing relationships between subunits and specific cis-acting motifs in protoplasts. Our results demonstrate that MED16, MED14, and MED2 are required for the full transcriptional activation by ABA of promoters containing both the ABRE (ABA-responsive element) and DRE (drought-responsive element). Using synthetic promoter motif concatamers, we showed that ABA-responsive activation of the ABRE but not the DRE motif was dependent on these three Mediator subunits. Furthermore, the three subunits were required for the control of water loss from leaves but played no role in ABA-dependent growth inhibition, highlighting specificity in their functions. Our results identify new roles for three Mediator subunits, provide a direct demonstration of their function and highlight that our experimental approach can be utilized to identify the function of subunits of plant transcriptional regulators.

Item Type: Journal Article
Subjects: Q Science > QH Natural history
Q Science > QK Botany
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Arabidopsis , Eukaryotic cells , Plant hormones -- Synthesis, Plant protoplasts
Journal or Publication Title: Frontiers in Plant Science
Publisher: Frontiers Media S.A.
ISSN: 1664-462X
Official Date: 11 March 2021
Dates:
DateEvent
11 March 2021Published
12 February 2021Accepted
Volume: 12
Article Number: 649720
DOI: 10.3389/fpls.2021.649720
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M017982/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/S012877/2[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDCommonwealth Scholarship Commissionhttp://dx.doi.org/10.13039/501100000867
BB-T008695-1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
1908125[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

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