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Condition-dependent transcriptional landscape of Campylobacter jejuni
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Lam, Jenna Hoi-Yan (2019) Condition-dependent transcriptional landscape of Campylobacter jejuni. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3501362~S15
Abstract
Campylobacter spp. are ubiquitous in the environment and can infect a plethora of animals, Campylobacter jejuni being the most predominant species infecting humans. One striking characteristic of this pathogen is its supposedly commensal status in avian species as opposed to an infectious agent in other animals. The main route of transmission to the human host is via consumption of undercooked poultry in developed countries, although there are other ways to become exposed such as through contact with infected animals or ingestion of contaminated water.
The sequenced reference strain NCTC 11168 revealed many features including a small tightly packed genome with three sigma factors and a limited number of global regulators. Furthermore, C. jejuni lacks many transcriptional factors present in other bacteria for rapid alteration of gene expression. Alternative forms of regulation such as small regulatory RNAs are not well characterised in C. jejuni, but may play a role in fine-tuning expression under different stresses. Studies in the literature have investigated the transcriptome of NCTC 11168 under standard growth or specific stress conditions, but this is the first time the transcriptional landscape of NCTC 11168 was determined under a collection of 22 host-relevant and transmission conditions, using a combination of high-throughput sequencing methodologies: RNAtag-Seq and Cappable-seq.
A highly reproducible data set representing the condition-dependent transcriptional landscape of NCTC 11168 was generated and assessed. This extensive data set includes gene expression profiles of individual conditions and the primary transcriptome of all combined conditions. A considerable repertoire of transcriptional start sites (TSS) were identified that corroborate with published data, with a significant number that were novel condition-dependent TSS and mainly internal. Preliminary analysis also revealed a subset of non-coding RNAs with an associated TSS. The behaviour of gene expression profiles was analysed and enriched for KEGG pathways with a focus on the behaviour of the stringent response. The data obtained from this project provides a good resource for the Campylobacter community, and is also a basis for future work to help decipher the regulatory systems of C. jejuni and give clues to how it can successfully act as a foodborne-pathogen.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
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Library of Congress Subject Headings (LCSH): | Campylobacter jejuni, Genetic transcription, Campylobacter infections, Food contamination, Food -- Microbiology | ||||
Official Date: | September 2019 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | School of Life Sciences | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Constantinidou, Chrystala ; Alam, Mohammad Tauqeer ; Denham, Emma | ||||
Sponsors: | Midlands Integrative Biosciences Training Partnership ; Biotechnology and Biological Sciences Research Council (Great Britain) | ||||
Format of File: | |||||
Extent: | xviii, 227 leaves : illustrations (some colour) | ||||
Language: | eng |
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