UNSPECIFIED (1998) Generation of an aroA mutant of Lactococcus lactis. Biotechnology Letters, 20 (10). pp. 917-921. ISSN 0141-5492

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Abstract

Oligonucleotide primers were designed from the DNA sequence of the aroA region from Lactococcus lactis and these were used to amplify regions adjacent to the aroA gene. The amplified fragments were cloned to produce a suicide plasmid vector for chromosomal integration. Transformation of L. lactis resulted in a single cross-over homologous recombination event and subsequent excision of the plasmid generated a strain lacking the aroA gene. Growth characteristics indicated that the mutant strain was deficient ire aroA.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology
Journal or Publication Title:	Biotechnology Letters
Publisher:	KLUWER ACADEMIC PUBL
ISSN:	0141-5492
Date:	October 1998
Volume:	20
Number:	10
Number of Pages:	5
Page Range:	pp. 917-921
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/15152

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