Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC

Tools
- Tools
+ Tools

Karlikowska, Magdalena, Singh, Albel, Bhatt, Apoorva, Ott, Sascha, Bottrill, Andrew R., Besra, Gurdyal S. and Fullam, Elizabeth (2021) Biochemical and phenotypic characterisation of the Mycobacterium smegmatis transporter UspABC. The Cell Surface, 7 . 100052. doi:10.1016/j.tcsw.2021.100052 ISSN 2468-2330.

[img]
Preview
PDF
WRAP-Biochemical-phenotypic-characterisation-mycobacterium-smegma‐2021.pdf - Accepted Version - Requires a PDF viewer.
Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0.

Download (4Mb) | Preview
Official URL: http://dx.doi.org/10.1016/j.tcsw.2021.100052

Request Changes to record.

Abstract

Mycobacterium tuberculosis (Mtb) is an intracellular human pathogen that has evolved to survive in a nutrient limited environment within the host for decades. Accordingly, Mtb has developed strategies to acquire scarce nutrients and the mycobacterial transporter systems provide an important route for the import of key energy sources. However, the physiological role of the Mtb transporters and their substrate preference(s) are poorly characterised. Previous studies have established that the Mtb UspC solute-binding domain recognises amino- and phosphorylated-sugars, indicating that the mycobacterial UspABC transporter plays a key role in the import of peptidoglycan precursors. Herein, we have used a wide array of approaches to investigate the role of UspABC in Mycobacterium smegmatis by analysis of mutant strains that either lack the solute binding domain: ΔuspC or the entire transport complex: ΔuspABC. Analysis of mycobacterial transcripts shows that the uspABC system is functionally expressed in mycobacteria as a contiguous reading frame. Topology mapping confirms an Nin-Cin orientation of the UspAB integral membrane spanning domains. Phenotypic microarray profiling of commercially available sugars suggests, unexpectedly, that the uspC and ΔuspABC mutants had different carbon utilisation profiles and that neither strain utilised glucose-1-phosphate. Furthermore, proteomics analysis showed an alteration in the abundance of proteins involved in sugar and lipid metabolism, crucial for cell envelope synthesis, and we propose that UspABC has an important role in determining the interplay between these pathways.

Item Type: Journal Article
Subjects: Q Science > QP Physiology
Q Science > QR Microbiology
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Mycobacteria , Mycobacterium tuberculosis, Tuberculosis -- Molecular aspects, Host-virus relationships, ATP-binding cassette transporters, Carrier proteins
Journal or Publication Title: The Cell Surface
Publisher: Elsevier
ISSN: 2468-2330
Official Date: December 2021
Dates:
DateEvent
December 2021Published
24 April 2021Available
3 April 2021Accepted
Volume: 7
Article Number: 100052
DOI: 10.1016/j.tcsw.2021.100052
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 4 May 2021
Date of first compliant Open Access: 24 April 2022
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
104193/Z/14/ZWellcome Trusthttp://dx.doi.org/10.13039/100010269
104193/Z/14/ZRoyal Societyhttp://dx.doi.org/10.13039/501100000288
RG120405Royal Societyhttp://dx.doi.org/10.13039/501100000288
BB/M01116X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M017982/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
Wolfson Research Merit AwardRoyal Societyhttp://dx.doi.org/10.13039/501100000288
MR/R001154/1 [MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MR/S000542/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
BB/L024209/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us