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Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
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Petre, Benjamin, Contreras, Mauricio P., Bozkurt, Tolga O., Schattat, Martin H., Sklenar, Jan, Schornack, Sebastian, Abd-El-Haliem, Ahmed, Castells-Graells, Roger, Lozano-Durán, Rosa, Dagdas, Yasin F., Menke, Frank L. H., Jones, Alexandra M. E., Vossen, Jack H., Robatzek, Silke, Kamoun, Sophien and Win, Joe (2021) Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. The Plant Cell, 33 (5). pp. 1447-1471. doi:10.1093/PLCELL/KOAB069 ISSN 1040-4651.
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WRAP-host-interactor-screens-phytophthora-infestans-RXLR-vesicle-trafficking-Jones-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3551Kb) | Preview |
Official URL: http://dx.doi.org/10.1093/PLCELL/KOAB069
Abstract
Pathogens modulate plant cell structure and function by secreting effectors into host tissues. Effectors typically function by associating with host molecules and modulating their activities. This study aimed to identify the host processes targeted by the RXLR class of host-translocated effectors of the potato blight pathogen Phytophthora infestans. To this end, we performed an in planta protein–protein interaction screen by transiently expressing P. infestans RXLR effectors in Nicotiana benthamiana leaves followed by coimmunoprecipitation and liquid chromatography-tandem mass spectrometry. This screen generated an effector–host protein interactome matrix of 59 P. infestans RXLR effectors x 586 N. benthamiana proteins. Classification of the host interactors into putative functional categories revealed over 35 biological processes possibly targeted by P. infestans. We further characterized the PexRD12/31 family of RXLR-WY effectors, which associate and colocalize with components of the vesicle trafficking machinery. One member of this family, PexRD31, increased the number of FYVE positive vesicles in N. benthamiana cells. FYVE positive vesicles also accumulated in leaf cells near P. infestans hyphae, indicating that the pathogen may enhance endosomal trafficking during infection. This interactome dataset will serve as a useful resource for functional studies of P. infestans effectors and of effector-targeted host processes.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | Q Science > QK Botany Q Science > QR Microbiology S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Phytophthora infestans, Phytopathogenic microorganisms , Phytopathogenic microorganisms -- Ecology, Phytopathogenic microorganisms -- Molecular aspect, Phytopathogenic microorganisms -- Host plants , Plant-pathogen relationships -- Molecular aspects | |||||||||||||||
| Journal or Publication Title: | The Plant Cell | |||||||||||||||
| Publisher: | American Society of Plant Biologists | |||||||||||||||
| ISSN: | 1040-4651 | |||||||||||||||
| Official Date: | May 2021 | |||||||||||||||
| Dates: |
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| Volume: | 33 | |||||||||||||||
| Number: | 5 | |||||||||||||||
| Page Range: | pp. 1447-1471 | |||||||||||||||
| DOI: | 10.1093/PLCELL/KOAB069 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 17 May 2021 | |||||||||||||||
| Date of first compliant Open Access: | 17 May 2021 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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