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Molecular identification and cellular localisation of a corticotropin-releasing hormone type neuropeptide in an echinoderm
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Cai, Weigang, Egertová, Michaela, Zampronio, Cleidiane G., Jones, Alexandra M. and Elphick, Maurice R. (2023) Molecular identification and cellular localisation of a corticotropin-releasing hormone type neuropeptide in an echinoderm. Neuroendocrinology, 113 (2). pp. 231-250. doi:10.1159/000517087 ISSN 0028-3835.
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WRAP-corticotropin-releasing-hormone-type-neuropeptide-echinoderm-Jones-2021.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3271Kb) | Preview |
Official URL: http://dx.doi.org/10.1159/000517087
Abstract
Background: Corticotropin-releasing hormone (CRH) mediates physiological responses to stressors in mammals by triggering pituitary secretion of adrenocorticotropic hormone, which stimulates adrenal release of cortisol. CRH belongs to a family of related neuropeptides that include sauvagine, urotensin-I and urocortins in vertebrates and the diuretic hormone DH44 in insects, indicating that the evolutionary origin of this neuropeptide family can be traced to the common ancestor of the Bilateria. However, little is known about CRH-type neuropeptides in deuterostome invertebrates. Methods: Here we used mass spectrometry, mRNA in situ hybridization and immunohistochemistry to investigate the structure and expression of a CRH-type neuropeptide (ArCRH) in the starfish Asterias rubens (phylum Echinodermata). Results: ArCRH is a 40-residue peptide with N-terminal pyroglutamylation and C-terminal amidation and it has a widespread pattern of expression in A. rubens. In the central nervous system comprising the circumoral nerve ring and five radial nerve cords, ArCRH-expressing cells and fibres were revealed in both the ectoneural region and the hyponeural region, which contains the cell bodies of motoneurons. Accordingly, ArCRH-immunoreactivity was detected in innervation of the ampulla and podium of locomotory organs (tube feet), and ArCRH is the first neuropeptide to be identified as a marker for nerve fibres located in the muscle layer of these organs. ArCRH-immunoreactivity was also revealed in protractile organs that mediate gas exchange (papulae), the apical muscle and the digestive system. Conclusions: Our findings provide the first insights into CRH-type neuropeptide expression and function in the unique context of the pentaradially symmetrical body plan of an echinoderm.
| Item Type: | Journal Article | ||||||||||||
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| Subjects: | Q Science > QL Zoology Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||
| Library of Congress Subject Headings (LCSH): | Corticotropin releasing hormone , Corticotropin releasing hormone -- Classification -- Molecular aspects, Neuropeptides , Echinodermata , Echinodermata -- Physiology | ||||||||||||
| Journal or Publication Title: | Neuroendocrinology | ||||||||||||
| Publisher: | Karger | ||||||||||||
| ISSN: | 0028-3835 | ||||||||||||
| Official Date: | February 2023 | ||||||||||||
| Dates: |
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| Volume: | 113 | ||||||||||||
| Number: | 2 | ||||||||||||
| Page Range: | pp. 231-250 | ||||||||||||
| DOI: | 10.1159/000517087 | ||||||||||||
| Status: | Peer Reviewed | ||||||||||||
| Publication Status: | Published | ||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||
| Date of first compliant deposit: | 17 May 2021 | ||||||||||||
| Date of first compliant Open Access: | 18 May 2021 | ||||||||||||
| RIOXX Funder/Project Grant: |
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