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The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish [pre-print]
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Vong, Yin Ho, Sivashanmugam, Lavanya, Zaucker, Andreas, Jones, Alex and Sampath, Karuna (2020) The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish [pre-print]. Working Paper. Cold Spring Harbor, New York: bioRxiv. (Unpublished)
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Official URL: http://dx.doi.org/10.1101/2020.06.23.167163
Abstract
The ability to reproduce is essential in all branches of life. In metazoans, this process is initiated by formation of the germline, a group of cells that are destined to form the future gonads, the tissue that will produce the gametes. The molecular mechanisms underlying germline formation differs between species. In zebrafish, development of the germline is dependent on the specification, migration and proliferation of progenitors called the primordial germ cells (PGCs). PGC specification is dependent on a maternally provided cytoplasmic complex of ribonucleoproteins (RNPs), the germplasm. Here, we show that the conserved RNA-binding protein (RBP), Igf2bp3, has an essential role during early embryonic development and germline development. Loss of Igf2bp3 leads to an expanded yolk syncytial layer (YSL) in early embryos, reduced germline RNA expression, and mis-regulated germline development. Maternal mutants affecting igf2bp3 exhibit abnormal PGCs and adult igf2bp3 mutants show male biased sex ratios. Therefore, Igf2bp3 is required for normal embryonic and germline development.
Item Type: | Working or Discussion Paper (Working Paper) | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Publisher: | bioRxiv | ||||
Place of Publication: | Cold Spring Harbor, New York | ||||
Official Date: | 24 June 2020 | ||||
Dates: |
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DOI: | 10.1101/2020.06.23.167163 | ||||
Institution: | University of Warwick | ||||
Status: | Not Peer Reviewed | ||||
Publication Status: | Unpublished | ||||
Access rights to Published version: | Open Access (Creative Commons) |
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