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A minimalistic cyclic ice-binding peptide from phage display
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Stevens, Corey A., Bachtiger, Fabienne, Kong, Xu-Dong, Abriata, Luciano A., Sosso, Gabriele C., Gibson, Matthew I. and Klok, Harm-Anton (2021) A minimalistic cyclic ice-binding peptide from phage display. Nature Communications, 12 (1). 2675. doi:10.1038/s41467-021-22883-w ISSN 2041-1723.
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Official URL: http://dx.doi.org/10.1038/s41467-021-22883-w
Abstract
Developing molecules that emulate the properties of naturally occurring ice-binding proteins (IBPs) is a daunting challenge. Rather than relying on the (limited) existing structure–property relationships that have been established for IBPs, here we report the use of phage display for the identification of short peptide mimics of IBPs. To this end, an ice-affinity selection protocol is developed, which enables the selection of a cyclic ice-binding peptide containing just 14 amino acids. Mutational analysis identifies three residues, Asp8, Thr10 and Thr14, which are found to be essential for ice binding. Molecular dynamics simulations reveal that the side chain of Thr10 hydrophobically binds to ice revealing a potential mechanism. To demonstrate the biotechnological potential of this peptide, it is expressed as a fusion (‘Ice-Tag’) with mCherry and used to purify proteins directly from cell lysate.
Item Type: | Journal Article | ||||||||||||||||||
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Subjects: | Q Science > QP Physiology | ||||||||||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Chemistry > Computational and Theoretical Chemistry Centre |
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Library of Congress Subject Headings (LCSH): | Antifreeze proteins | ||||||||||||||||||
Journal or Publication Title: | Nature Communications | ||||||||||||||||||
Publisher: | Nature Publishing Group | ||||||||||||||||||
ISSN: | 2041-1723 | ||||||||||||||||||
Official Date: | 11 May 2021 | ||||||||||||||||||
Dates: |
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Volume: | 12 | ||||||||||||||||||
Number: | 1 | ||||||||||||||||||
Article Number: | 2675 | ||||||||||||||||||
DOI: | 10.1038/s41467-021-22883-w | ||||||||||||||||||
Status: | Peer Reviewed | ||||||||||||||||||
Publication Status: | Published | ||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||||||||||
Date of first compliant deposit: | 18 May 2021 | ||||||||||||||||||
Date of first compliant Open Access: | 19 May 2021 | ||||||||||||||||||
RIOXX Funder/Project Grant: |
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