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Miniaturized probe on polymer SU-8 with array of individually addressable microelectrodes for electrochemical analysis in neural and other biological tissues
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Lotfi Marchoubeh, Mahsa, Cobb, Samuel J., Abrego Tello, Miguel, Hu, Mengjia, Jaquins-Gerstl, Andrea, Robbins, Elaine M., Macpherson, Julie V., Michael, Adrian C. and Fritsch, Ingrid (2021) Miniaturized probe on polymer SU-8 with array of individually addressable microelectrodes for electrochemical analysis in neural and other biological tissues. Analytical and Bioanalytical Chemistry, 413 . pp. 6777-6791. doi:10.1007/s00216-021-03327-2 ISSN 1618-2642.
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Official URL: http://dx.doi.org/10.1007/s00216-021-03327-2
Abstract
An SU-8 probe with an array of nine, individually addressable gold microband electrodes (100 μm long, 4 μm wide, separated by 4-μm gaps) was photolithographically fabricated and characterized for detection of low concentrations of chemicals in confined spaces and in vivo studies of biological tissues. The probe's shank (6 mm long, 100 μm wide, 100 μm thick) is flexible, but exhibits sufficient sharpness and rigidity to be inserted into soft tissue. Laser micromachining was used to define probe geometry by spatially revealing the underlying sacrificial aluminum layer, which was then etched to free the probes from a silicon wafer. Perfusion with fluorescent nanobeads showed that, like a carbon fiber electrode, the probe produced no noticeable damage when inserted into rat brain, in contrast to damage from an inserted microdialysis probe. The individual addressability of the electrodes allows single and multiple electrode activation. Redox cycling is possible, where adjacent electrodes serve as generators (that oxidize or reduce molecules) and collectors (that do the opposite) to amplify signals of small concentrations without background subtraction. Information about electrochemical mechanisms and kinetics may also be obtained. Detection limits for potassium ferricyanide in potassium chloride electrolyte of 2.19, 1.25, and 2.08 μM and for dopamine in artificial cerebral spinal fluid of 1.94, 1.08, and 5.66 μM for generators alone and for generators and collectors during redox cycling, respectively, were obtained.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Journal or Publication Title: | Analytical and Bioanalytical Chemistry | ||||||||
Publisher: | Springer | ||||||||
ISSN: | 1618-2642 | ||||||||
Official Date: | November 2021 | ||||||||
Dates: |
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Volume: | 413 | ||||||||
Page Range: | pp. 6777-6791 | ||||||||
DOI: | 10.1007/s00216-021-03327-2 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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