Skip to content Skip to navigation
University of Warwick
  • Study
  • |
  • Research
  • |
  • Business
  • |
  • Alumni
  • |
  • News
  • |
  • About

University of Warwick
Publications service & WRAP

Highlight your research

  • WRAP
    • Home
    • Search WRAP
    • Browse by Warwick Author
    • Browse WRAP by Year
    • Browse WRAP by Subject
    • Browse WRAP by Department
    • Browse WRAP by Funder
    • Browse Theses by Department
  • Publications Service
    • Home
    • Search Publications Service
    • Browse by Warwick Author
    • Browse Publications service by Year
    • Browse Publications service by Subject
    • Browse Publications service by Department
    • Browse Publications service by Funder
  • Help & Advice
University of Warwick

The Library

  • Login
  • Admin

Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis

Tools
- Tools
+ Tools

Pardal, Alonso J., Piquerez, Sophie J. M., Dominguez-Ferreras, Ana, Frungillo, Lucas, Mastorakis, Emmanouil, Reilly, Emma, Latrasse, David, Concia, Lorenzo, Gimenez-Ibanez, Selena, Spoel, Steven H., Benhamed, Moussa and Ntoukakis, Vardis (2021) Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis. PLOS Pathogens, 17 (5). e1009572. doi:10.1371/journal.ppat.1009572

[img]
Preview
PDF
WRAP-Immunity-onset-plant-chromatin-homeostasis-2021.pdf - Published Version - Requires a PDF viewer.
Available under License Creative Commons Attribution 4.0.

Download (2186Kb) | Preview
Official URL: https://doi.org/10.1371/journal.ppat.1009572

Request Changes to record.

Abstract

Perception of microbes by plants leads to dynamic reprogramming of the transcriptome, which is essential for plant health. The appropriate amplitude of this transcriptional response can be regulated at multiple levels, including chromatin. However, the mechanisms underlying the interplay between chromatin remodeling and transcription dynamics upon activation of plant immunity remain poorly understood. Here, we present evidence that activation of plant immunity by bacteria leads to nucleosome repositioning, which correlates with altered transcription. Nucleosome remodeling follows distinct patterns of nucleosome repositioning at different loci. Using a reverse genetic screen, we identify multiple chromatin remodeling ATPases with previously undescribed roles in immunity, including EMBRYO SAC DEVELOPMENT ARREST 16, EDA16. Functional characterization of the immune-inducible chromatin remodeling ATPase EDA16 revealed a mechanism to negatively regulate immunity activation and limit changes in redox homeostasis. Our transcriptomic data combined with MNase-seq data for EDA16 functional knock-out and over-expressor mutants show that EDA16 selectively regulates a defined subset of genes involved in redox signaling through nucleosome repositioning. Thus, collectively, chromatin remodeling ATPases fine-tune immune responses and provide a previously uncharacterized mechanism of immune regulation.

Item Type: Journal Article
Subjects: Q Science > QH Natural history > QH426 Genetics
S Agriculture > SB Plant culture
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): Plant immunology, Plants -- Disease and pest resistance, Chromatin -- Structure
Journal or Publication Title: PLOS Pathogens
Publisher: Public Library of Science
ISSN: 1553-7374
Official Date: 20 May 2021
Dates:
DateEvent
20 May 2021Published
19 April 2021Accepted
Volume: 17
Number: 5
Article Number: e1009572
DOI: 10.1371/journal.ppat.1009572
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/M01116X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/M017982/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIED[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
UF160546[RS] Royal Societyhttp://dx.doi.org/10.13039/501100000288
678511H2020 European Research Councilhttp://dx.doi.org/10.13039/100010663
UNSPECIFIED[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
Related URLs:
  • http://creativecommons.org/licenses/by/4...
Contributors:
ContributionNameContributor ID
UNSPECIFIEDMackey, DavidUNSPECIFIED

Request changes or add full text files to a record

Repository staff actions (login required)

View Item View Item

Downloads

Downloads per month over past year

View more statistics

twitter

Email us: wrap@warwick.ac.uk
Contact Details
About Us