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Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis
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Pardal, Alonso J., Piquerez, Sophie J. M., Dominguez-Ferreras, Ana, Frungillo, Lucas, Mastorakis, Emmanouil, Reilly, Emma, Latrasse, David, Concia, Lorenzo, Gimenez-Ibanez, Selena, Spoel, Steven H., Benhamed, Moussa and Ntoukakis, Vardis (2021) Immunity onset alters plant chromatin and utilizes EDA16 to regulate oxidative homeostasis. PLOS Pathogens, 17 (5). e1009572. doi:10.1371/journal.ppat.1009572 ISSN 1553-7374.
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WRAP-Immunity-onset-plant-chromatin-homeostasis-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2186Kb) | Preview |
Official URL: https://doi.org/10.1371/journal.ppat.1009572
Abstract
Perception of microbes by plants leads to dynamic reprogramming of the transcriptome, which is essential for plant health. The appropriate amplitude of this transcriptional response can be regulated at multiple levels, including chromatin. However, the mechanisms underlying the interplay between chromatin remodeling and transcription dynamics upon activation of plant immunity remain poorly understood. Here, we present evidence that activation of plant immunity by bacteria leads to nucleosome repositioning, which correlates with altered transcription. Nucleosome remodeling follows distinct patterns of nucleosome repositioning at different loci. Using a reverse genetic screen, we identify multiple chromatin remodeling ATPases with previously undescribed roles in immunity, including EMBRYO SAC DEVELOPMENT ARREST 16, EDA16. Functional characterization of the immune-inducible chromatin remodeling ATPase EDA16 revealed a mechanism to negatively regulate immunity activation and limit changes in redox homeostasis. Our transcriptomic data combined with MNase-seq data for EDA16 functional knock-out and over-expressor mutants show that EDA16 selectively regulates a defined subset of genes involved in redox signaling through nucleosome repositioning. Thus, collectively, chromatin remodeling ATPases fine-tune immune responses and provide a previously uncharacterized mechanism of immune regulation.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history > QH426 Genetics S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| SWORD Depositor: | Library Publications Router | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Plant immunology, Plants -- Disease and pest resistance, Chromatin -- Structure | |||||||||||||||||||||
| Journal or Publication Title: | PLOS Pathogens | |||||||||||||||||||||
| Publisher: | Public Library of Science | |||||||||||||||||||||
| ISSN: | 1553-7374 | |||||||||||||||||||||
| Official Date: | 20 May 2021 | |||||||||||||||||||||
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| Volume: | 17 | |||||||||||||||||||||
| Number: | 5 | |||||||||||||||||||||
| Article Number: | e1009572 | |||||||||||||||||||||
| DOI: | 10.1371/journal.ppat.1009572 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 9 June 2021 | |||||||||||||||||||||
| Date of first compliant Open Access: | 9 June 2021 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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| Is Part Of: | 1 | |||||||||||||||||||||
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