The Library
The polymeric glyco-linker controls the signal outputs for plasmonic gold nanorod biosensors due to biocorona formation
Tools
Tools
Tools
Pancaro, Alessia, Szymonik, Michal, Georgiou, Panagiotis G., Baker, Alexander N., Walker, Marc, Adriaensens, Peter, Hendrix, Jelle, Gibson, Matthew I. and Nelissen, Inge (2021) The polymeric glyco-linker controls the signal outputs for plasmonic gold nanorod biosensors due to biocorona formation. Nanoscale, 13 (24). pp. 10837-10848. doi:10.1039/D1NR01548F
|
PDF
WRAP-polymeric-glyco-linker-controls-signal-outputs-plasmonic-gold-nanorod-biosensors-due-biocorona-formation-Gibson-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (2813Kb) | Preview |
Official URL: http://dx.doi.org/10.1039/D1NR01548F
Abstract
Gold nanorods (GNRs) are a promising platform for nanoplasmonic biosensing. The localised surface plasmon resonance (LSPR) peak of GNRs is located in the near-infrared optical window and is sensitive to local binding events, enabling label-free detection of biomarkers in complex biological fluids. A key challenge in the development of such sensors is achieving target affinity and selectivity, while both minimizing non-specific binding and maintaining colloidal stability. Herein, we reveal how GNRs decorated with galactosamine-terminated polymer ligands display significantly different binding responses in buffer compared to serum, due to biocorona formation, and how biocorona displacement due to lectin binding plays a key role in their optical responses. GNRs were coated with either poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) or poly(N-hydroxyethyl acrylamide) (PHEA) prepared via reversible addition–fragmentation chain-transfer (RAFT) polymerisation and end-functionalised with galactosamine (Gal) as the lectin-targeting unit. In buffer Gal-PHEA-coated GNRs aggregated upon soybean agglutinin (SBA) addition, whereas Gal-PHPMA-coated GNRs exhibited a red-shift of the LSPR spectrum without aggregation. In contrast, when incubated in serum Gal-PHPMA-coated nanorods showed no binding response, while Gal-PHEA GNRs exhibited a dose-dependent blue-shift of the LSPR peak, which is the opposite direction (red-shift) to what was observed in buffer. This differential behaviour was attributed to biocorona formation onto both polymer-coated GNRs, shown by differential centrifugal sedimentation and nanoparticle tracking analysis. Upon addition of SBA to the Gal-PHEA coated nanorods, signal was generated due to displacement of weakly-bound biocorona components by lectin binding. However, in the case of Gal-PHPMA which had a thicker corona, attributed to lower polymer grafting densities, addition of SBA did not lead to biocorona displacement and there was no signal output. These results show that plasmonic optical responses in complex biological media can be significantly affected by biocorona formation, and that biocorona formation itself does not prevent sensing so long as its exact nature (e.g. ‘hard versus soft’) is tuned.
| Item Type: | Journal Article | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QC Physics Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology T Technology > TA Engineering (General). Civil engineering (General) |
||||||||||||||||||
| Divisions: | Faculty of Science > Chemistry | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Nanoparticles, Surface plasmon resonance, Biochemical markers , Lectins , Polymerization | ||||||||||||||||||
| Journal or Publication Title: | Nanoscale | ||||||||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||||||||
| ISSN: | 2040-3364 | ||||||||||||||||||
| Official Date: | 28 June 2021 | ||||||||||||||||||
| Dates: |
|
||||||||||||||||||
| Volume: | 13 | ||||||||||||||||||
| Number: | 24 | ||||||||||||||||||
| Page Range: | pp. 10837-10848 | ||||||||||||||||||
| DOI: | 10.1039/D1NR01548F | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
