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A multi-omics approach in the characterisation of the RNA-binding protein SpoVG in Bacillus subtilis
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Mahapatra, Swati (2020) A multi-omics approach in the characterisation of the RNA-binding protein SpoVG in Bacillus subtilis. PhD thesis, University of Warwick.
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Official URL: http://webcat.warwick.ac.uk/record=b3598160~S15
Abstract
Post-transcriptional gene regulation via small non-coding RNAs (sRNAs) have been well characterised in many bacteria. RNA-binding proteins (RBPs) are emerging as key players in mediating sRNA based gene regulation. Most of these studies have focused on investigating Gram-negative bacteria. Nonetheless, little is known about RBPs in Gram-positive bacteria. Furthermore, in many Gram-positive bacteria the known RBPs are either absent or do not function similar to RBPs in the Gram-negatives. Such evidence suggest alternate gene regulatory mechanisms in Gram-positives that either include alternative RBPs or regulatory pathways that do not involve RBPs. Emerging studies in various bacteria have identified SpoVG as a novel RBP. spoVG was initially identified in B. subtilis as a sporulation gene. The gene is highly conserved with constitutive expression in B. subtilis. However, nothing is known about its RNA-binding activities in B. subtilis.
In this thesis, it was hypothesised that SpoVG is an RBP in vivo and a post-transcriptional gene regulator in B. subtilis. To test this, a global multi-omics approach was taken. It was shown that deletion of spoVG perturbs both the total transcriptomic and proteomic landscapes of B. subtilis (Chapters 3 and 4). SpoVG was also identified as a global RBP (Chapter 5). A protein-protein interaction study indicated potential protein binding activity of SpoVG (Chapter 6). Finally, ∆spoVG was functionally characterised based on the - omics data from this thesis and novel phenotypes of ∆spoVG were reported (Chapter 7). Cumulatively, these results indicated a global regulatory role of SpoVG in B. subtilis beyond its role in sporulation. Nevertheless, its role as a global post-transcriptional gene regulator remains elusive. The findings reported in this study not only promote SpoVG as novel RBP with regulator functions in B. subtilis, but they will also benefit in uncovering the less explored realms of regulatory networks of RBPs in other bacteria.
Item Type: | Thesis (PhD) | ||||
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Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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Library of Congress Subject Headings (LCSH): | RNA-protein interactions, Gram-positive bacteria, Genetic regulation, Bacillus subtilis | ||||
Official Date: | November 2020 | ||||
Dates: |
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Institution: | University of Warwick | ||||
Theses Department: | Warwick Medical School | ||||
Thesis Type: | PhD | ||||
Publication Status: | Unpublished | ||||
Supervisor(s)/Advisor: | Denham, Emma ; Constantinidou, Chrystala | ||||
Sponsors: | University of Warwick | ||||
Format of File: | |||||
Extent: | xvii, 285 leaves : illustrations (chiefly colour) | ||||
Language: | eng |
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