The Library
Sodium in the dermis colocates to glycosaminoglycan scaffold, with diminishment in type 2 diabetes mellitus
Tools
Tools
Tools
Hanson, Petra, Philp, Christopher J., Randeva, Harpal S., James, Sean G., O’Hare, Paul, Meersmann, Thomas, Pavlovskaya, Galina E. and Barber, Thomas M. (2021) Sodium in the dermis colocates to glycosaminoglycan scaffold, with diminishment in type 2 diabetes mellitus. JCI Insight, 6 (12). e145470. doi:10.1172/jci.insight.145470 ISSN 2379-3708.
|
PDF
WRAP-sodium-dermis-colocates-glycosaminoglycan-scaffold-diminishment-type-2-diabetes-mellitus-Barber-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (6Mb) | Preview |
Official URL: http://dx.doi.org/10.1172/jci.insight.145470
Abstract
BACKGROUND. Dietary sodium intake mismatches urinary sodium excretion over prolonged periods. Our aims were to localize and quantify electrostatically bound sodium within human skin using triple-quantum–filtered (TQF) protocols for MRI and magnetic resonance spectroscopy (MRS) and to explore dermal sodium in type 2 diabetes mellitus (T2D).
METHODS. We recruited adult participants with T2D (n = 9) and euglycemic participants with no history of diabetes mellitus (n = 8). All had undergone lower limb amputations or abdominal skin reduction surgery for clinical purposes. We used 20 μm in-plane resolution 1H MRI to visualize anatomical skin regions ex vivo from skin biopsies taken intraoperatively, 23Na TQF MRI/MRS to explore distribution and quantification of freely dissolved and bound sodium, and inductively coupled plasma mass spectrometry to quantify sodium in selected skin samples.
RESULTS. Human dermis has a preponderance (>90%) of bound sodium that colocalizes with the glycosaminoglycan (GAG) scaffold. Bound and free sodium have similar anatomical locations. T2D associates with a severely reduced dermal bound sodium capacity.
CONCLUSION. We provide the first evidence to our knowledge for high levels of bound sodium within human dermis, colocating to the GAG scaffold, consistent with a dermal “third space repository” for sodium. T2D associates with diminished dermal electrostatic binding capacity for sodium.
| Item Type: | Journal Article | ||||||
|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QM Human anatomy Q Science > QP Physiology R Medicine > RC Internal medicine |
||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||
| Library of Congress Subject Headings (LCSH): | Dermis , Sodium , Sodium -- Physiological effect , Sodium in the body , Non-insulin-dependent diabetes -- Treatment | ||||||
| Journal or Publication Title: | JCI Insight | ||||||
| Publisher: | American Society for Clinical Investigation | ||||||
| ISSN: | 2379-3708 | ||||||
| Official Date: | 22 June 2021 | ||||||
| Dates: |
|
||||||
| Volume: | 6 | ||||||
| Number: | 12 | ||||||
| Article Number: | e145470 | ||||||
| DOI: | 10.1172/jci.insight.145470 | ||||||
| Status: | Peer Reviewed | ||||||
| Publication Status: | Published | ||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||
| Date of first compliant deposit: | 25 June 2021 | ||||||
| Date of first compliant Open Access: | 25 June 2021 | ||||||
| RIOXX Funder/Project Grant: |
|
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
Downloads
Downloads per month over past year
