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Chemical synthesis of globotriose and galabiose: relative stabilities of their complexes with Escherichia coli Shiga-like toxin-1 as determined by denaturation-titration with guanidinium chloride
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UNSPECIFIED (1998) Chemical synthesis of globotriose and galabiose: relative stabilities of their complexes with Escherichia coli Shiga-like toxin-1 as determined by denaturation-titration with guanidinium chloride. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 (15). pp. 2287-2294. ISSN 0300-922X
Full text not available from this repository.Abstract
Globotriose [alpha-D-Gal-(1-->4)-beta-D-Gal-(1-->4)-D-Glc] is the carbohydrate moiety of the globotriosyl ceramide (Gb(3)), also known as the germinal centre B-cell differentiation antigen CD77, a glycolipid present on the plasma membrane of certain mammalian cells. In Gb(3), globotriose functions as the cell-surface receptor for Shiga toxin and for the Shiga-like toxins (verocytotoxins). Here we report the chemical synthesis of globotriose and the corresponding terminal disaccharide, galabiose [alpha-D-Gal(1-->4)-beta-D-Gal]. Globotriose and galabiose are attached via a linker to CNBr-activated Sepharose to generate affinity matrices that permit the one-step purification of recombinant Shiga-like toxin-l from crude E. coli homogenates. Toxin is released from either of the immobilised saccharides by elution with 6 M guanidinium chloride. After dilution of the denaturant, the released toxin had full catalytic activity. Denaturation-titration experiments show that the bound toxin is released from galabiose-Sepharose at 2.3 M guanidinium chloride, while its release from globotriose-Sepharose requires a higher concentration of 4.8 M. These results indicate that the glucose component of globotriose contributes similar to 2.6 kcal mol(-1) to the binding;energy relative to galabiose.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Journal or Publication Title: | JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 |
| Publisher: | ROYAL SOC CHEMISTRY |
| ISSN: | 0300-922X |
| Date: | 7 August 1998 |
| Number: | 15 |
| Number of Pages: | 8 |
| Page Range: | pp. 2287-2294 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/15465 |
Data sourced from Thomson Reuters' Web of Knowledge
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