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Assessment strategy for bacterial lignin depolymerization : Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
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Rouches, E., Gómez-Alvarez, H., Majira, A., Martín-Moldes, Z., Nogales, J., Díaz, E., Bugg, Tim and Baumberger, S. (2021) Assessment strategy for bacterial lignin depolymerization : Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida. Bioresource Technology Reports, 15 . 100742. doi:10.1016/j.biteb.2021.100742 ISSN 2589-014X.
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WRAP-Assessment-strategy-bacterial-lignin-depolymerization-Kraft-lignin-synthetic-lignin-bioconversion-Pseudomonas-2021.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (491Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.biteb.2021.100742
Abstract
In order to better understand bacterial depolymerization of lignin, a new analytical approach was proposed using Pseudomonas putida KT2440 as delignifying bacterium and Escherichia coli as non-delignifying control. Two different types of lignins, technical Kraft lignin and synthetic dehydrogenopolymer (DHP), were submitted to a bioconversion kinetic study over 7 days. The concomitant analysis of the supernatant acid-precipitable lignin fraction and water-soluble extractives by high-performance size-exclusion chromatography (HPSEC) and gas chromatography – mass spectrometry (GC–MS) highlighted the specific action of P. putida towards these substrates, with the transitory formation of phenolic metabolites (dihydroferulic acid for Kraft lignins and dimers for DHP) and the prevention of Kraft lignin self-assemblying. In both cases lignin apparent depolymerization followed by repolymerization was observed. The analysis of the bacterial pellets indicated the time-increasing content of lignins associated to bacterial cells, which could account for the apparent structural changes observed with E. coli in the supernatant.
Item Type: | Journal Article | |||||||||||||||
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Subjects: | Q Science > QD Chemistry Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||
Library of Congress Subject Headings (LCSH): | Polymerization , Lignin , Phenols | |||||||||||||||
Journal or Publication Title: | Bioresource Technology Reports | |||||||||||||||
Publisher: | Elsevier | |||||||||||||||
ISSN: | 2589-014X | |||||||||||||||
Official Date: | September 2021 | |||||||||||||||
Dates: |
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Volume: | 15 | |||||||||||||||
Article Number: | 100742 | |||||||||||||||
DOI: | 10.1016/j.biteb.2021.100742 | |||||||||||||||
Status: | Peer Reviewed | |||||||||||||||
Publication Status: | Published | |||||||||||||||
Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||
Date of first compliant deposit: | 13 July 2021 | |||||||||||||||
Date of first compliant Open Access: | 22 June 2022 | |||||||||||||||
RIOXX Funder/Project Grant: |
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