Overlapping functions of components of a bacterial Sec-independent protein export pathway
UNSPECIFIED. (1998) Overlapping functions of components of a bacterial Sec-independent protein export pathway. EMBO JOURNAL, 17 (13). pp. 3640-3650. ISSN 0261-4189Full text not available from this repository.
We describe the identification of two Escherichia coli genes required for the export of cofactor-containing periplasmic proteins, synthesized with signal peptides containing a twin arginine motif, Both gene products are homologous to the maize HCF106 protein required for the translocation of a subset of lumenal proteins across the thylakoid membrane. Disruption of either gene affects the export of a range of such proteins, and a complete block is observed when both genes are inactivated. The Sec protein export pathway was unaffected, indicating the involvement of the gene products in a novel export system. The accumulation of active cofactor-containing proteins in the cytoplasm of the mutant strains suggests a role for the gene products in the translocation of folded proteins. One of the two HCF106 homologues is encoded by the first gene of a four cistron operon, tatABCD, and the second by an unlinked gene, tatE, A mutation previously assigned to the hcf106 homologue encoded at the tatABCD locus, mttA, lies instead in the tatB gene.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||EMBO JOURNAL|
|Publisher:||OXFORD UNIV PRESS|
|Date:||1 July 1998|
|Number of Pages:||11|
|Page Range:||pp. 3640-3650|
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