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Detection of AtRKD4 protein during induction of somatic embryogenesis in Dendrobium lineale Rolfe transgenic orchids carrying 35S::GR::AtRKD4

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Wirajagat, Gde Cahyadi, Febryanti, Ni Luh Putu Kayika, Puspitasari, Fitriana, Sundari, Dika, Gutierrez-Marcos, José F. and Semiarti, Endang (2021) Detection of AtRKD4 protein during induction of somatic embryogenesis in Dendrobium lineale Rolfe transgenic orchids carrying 35S::GR::AtRKD4. Journal of Tropical Biodiversity and Biotechnology, 6 (2). 61783. doi:10.22146/jtbb.61783

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Official URL: http://dx.doi.org/10.22146/jtbb.61783

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Abstract

Dendrobium lineale is an Indonesian native orchid from the Spatulata section in Orchidaceae Family. This orchid is important because it is usually used as a parental plant in orchid breeding and is predicted to have a potential phytochemistry compound. In addition, in their natural habitat, this orchid is threatened due to forest exploitation and natural disaster. Therefore the precision mass propagation techniques for this orchid need to be conducted. Biotechnological approaches through inserting embryo gene such as AtRKD4 from Arabidopsis thaliana has already been successfully conducted. This study aims to check the integration stability of T-DNA harboring 35S::GR::AtRKD4 from ten selection transformants and to detect the existence of AtRKD4 protein after induction by Dexamethasone and/ Thidiazuron. The result showed that T-DNA were stably integrated into the genome of D. lineale transformants and the AtRKD4 protein with a molecular weight of 28.53 kDa was detected in D. lineale transformant plants after being induced by 15 µM DEX and 3 mgL-1 TDZ for 5 days.

Item Type: Journal Article
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Orchids, Dendrobium lineale, Orchids -- Biotechnology, Botany, Experimental, Plant physiology -- Genetic aspects
Journal or Publication Title: Journal of Tropical Biodiversity and Biotechnology
ISSN: 2540-9573
Official Date: 4 June 2021
Dates:
DateEvent
4 June 2021Published
29 April 2021Accepted
Volume: 6
Number: 2
Article Number: 61783
DOI: 10.22146/jtbb.61783
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDMinistry of Research and Technology of the Republic of IndonesiaUNSPECIFIED

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