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Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations
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Dorning, Ashley, Dhami, Priya, Panir, Kavita, Hogg, Chloe, Park, Emma, Ferguson, Gregory D., Hargrove, Diane, Karras, James, Horne, Andrew W. and Greaves, Erin (2021) Bioluminescent imaging in induced mouse models of endometriosis reveals differences in four model variations. Disease Models and Mechanisms, 14 (8). dmm049070. doi:10.1242/dmm.049070
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WRAP-bioluminescent-imaging-induced-mouse-models-endometriosis-reveals-differences-four-model-variations-Greaves-2021.pdf - Accepted Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2293Kb) | Preview |
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WRAP-bioluminescent-imaging-induced-mouse-models-endometriosis-reveals-differences-four-model-variations-Greaves-2021.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (1993Kb) |
Official URL: https://doi.org/10.1242/dmm.049070
Abstract
Our understanding of the etiology and pathophysiology of endometriosis remains limited. Disease modelling in the field is problematic as many versions of induced mouse models of endometriosis exist. We integrated bioluminescent imaging of ‘lesions’ generated using luciferase-expressing donor mice. We compared longitudinal bioluminescence and histology of lesions, sensory behavior of mice with induced endometriosis and the impact of the GnRH antagonist Cetrorelix on lesion regression and sensory behavior. Four models of endometriosis were tested. We found that the nature of the donor uterine material was a key determinant of how chronic the lesions were as well as their cellular composition. The severity of pain-like behavior also varied across models. Whilst Cetrorelix significantly reduced lesion bioluminescence in all models, it had varying impacts on pain-like behavior. Collectively, our results demonstrate key differences in the progression of the ‘disease’ across different mouse models of endometriosis. We propose that validation and testing in multiple models, each of which may be representative of the different subtypes / heterogeneity observed in women should become a standard approach to discovery science in the field of endometriosis.
| Item Type: | Journal Article | |||||||||
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| Subjects: | R Medicine > RG Gynecology and obstetrics | |||||||||
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences Faculty of Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Endometriosis -- Etiology -- Research, Bioluminescence, Imaging systems in biology -- Methodology | |||||||||
| Journal or Publication Title: | Disease Models and Mechanisms | |||||||||
| Publisher: | The Company of Biologists | |||||||||
| ISSN: | 1754-8403 | |||||||||
| Official Date: | 12 August 2021 | |||||||||
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| Volume: | 14 | |||||||||
| Number: | 8 | |||||||||
| Article Number: | dmm049070 | |||||||||
| DOI: | 10.1242/dmm.049070 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access | |||||||||
| RIOXX Funder/Project Grant: |
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