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Evolutionary trajectory of the replication mode of bacterial replicons
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Xie, Bin-Bin, Rong, Jin-Cheng, Tang, Bai-Lu, Wang, Sishuo, Liu, Guiming, Qin, Qi-Long, Zhang, Xi-Ying, Zhang, Weipeng, She, Qunxin, Chen, Yin, Li, Fuchuan, Li, Shengying, Chen, Xiu-Lan, Luo, Haiwei, Zhang, Yu-zhong and Cooper, Vaughn S. (2021) Evolutionary trajectory of the replication mode of bacterial replicons. mBio, 12 (1). e02745-20. doi:10.1128/mBio.02745-20
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WRAP-evolutionary-trajectory-replication-mode-bacterial-replicons-Chen-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2124Kb) | Preview |
Official URL: http://dx.doi.org/10.1128/mBio.02745-20
Abstract
As typical bacterial replicons, circular chromosomes replicate bidirectionally and circular plasmids replicate either bidirectionally or unidirectionally. Whereas the finding of chromids (plasmid-derived chromosomes) in multiple bacterial lineages provides circumstantial evidence that chromosomes likely evolved from plasmids, all experimentally assayed chromids were shown to use bidirectional replication. Here, we employed a model system, the marine bacterial genus Pseudoalteromonas, members of which consistently carry a chromosome and a chromid. We provide experimental and bioinformatic evidence that while chromids in a few strains replicate bidirectionally, most replicate unidirectionally. This is the first experimental demonstration of the unidirectional replication mode in bacterial chromids. Phylogenomic and comparative genomic analyses showed that the bidirectional replication evolved only once from a unidirectional ancestor and that this transition was associated with insertions of exogenous DNA and relocation of the replication terminus region (ter2) from near the origin site (ori2) to a position roughly opposite it. This process enables a plasmid-derived chromosome to increase its size and expand the bacterium’s metabolic versatility while keeping its replication synchronized with that of the main chromosome. A major implication of our study is that the uni- and bidirectionally replicating chromids may represent two stages on the evolutionary trajectory from unidirectionally replicating plasmids to bidirectionally replicating chromosomes in bacteria. Further bioinformatic analyses predicted unidirectionally replicating chromids in several unrelated bacterial phyla, suggesting that evolution from unidirectionally to bidirectionally replicating replicons occurred multiple times in bacteria.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history | |||||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Chromosome replication , Plasmids , Bacterial chromosomes | |||||||||||||||||||||||||||||||||
| Journal or Publication Title: | mBio | |||||||||||||||||||||||||||||||||
| Publisher: | American Society for Microbiology | |||||||||||||||||||||||||||||||||
| ISSN: | 2150-7511 | |||||||||||||||||||||||||||||||||
| Official Date: | 26 January 2021 | |||||||||||||||||||||||||||||||||
| Dates: |
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| Volume: | 12 | |||||||||||||||||||||||||||||||||
| Number: | 1 | |||||||||||||||||||||||||||||||||
| Article Number: | e02745-20 | |||||||||||||||||||||||||||||||||
| DOI: | 10.1128/mBio.02745-20 | |||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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