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Sequence-alignment modelling and molecular docking studies of the epoxygenase component of alkene monooxygenase from Nocardia corallina B-276
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UNSPECIFIED (1998) Sequence-alignment modelling and molecular docking studies of the epoxygenase component of alkene monooxygenase from Nocardia corallina B-276. EUROPEAN JOURNAL OF BIOCHEMISTRY, 254 (3). pp. 480-489. ISSN 0014-2956
Full text not available from this repository.Abstract
Whole cells of Nocardia corallina B-276 catalyse the stereoselective epoxygenation of alkenes to chiral epoxides. The bacterium expresses an enzyme, alkene monooxygenase, which catalyses the epoxygenation reaction stereoselectively. The enzyme consists of a terminal oxygenase (epoxygenase), an NADH-dependent reductase (reductase) and a regulatory component (coupling protein). The epoxygenase component contains a bridged diiron centre similar to that found in the hydroxylase component of soluble methane monooxygenase. Sequence-alignment modelling, supported by chemical modification and fluorescence probing, identified a hydrophobic oxygen/substrate binding site within the epoxygenase. The diiron centre was coordinated by the two His and two Glu residues from two conserved Glu-Xaa-Xaa-His sequences and by two further Glu residues. Molecular docking of substrates and products into the proposed active-site model of the epoxygenase suggested that Ala91 and Ala185 were responsible for the stereoselectivity exerted by AMO. It is proposed that these residues clamped the intermediate and/or product of the reaction, thereby controlling the configuration of the epoxide produced. In soluble methane monooxygenase these residues are replaced by two Gly residues which do not provide sufficient steric hindrance to prevent rotation of the intermediate in the active site and, therefore, the product of the reaction catalysed by this enzyme is achiral.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QD Chemistry |
| Journal or Publication Title: | EUROPEAN JOURNAL OF BIOCHEMISTRY |
| Publisher: | SPRINGER VERLAG |
| ISSN: | 0014-2956 |
| Date: | 15 June 1998 |
| Volume: | 254 |
| Number: | 3 |
| Number of Pages: | 10 |
| Page Range: | pp. 480-489 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/15616 |
Data sourced from Thomson Reuters' Web of Knowledge
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