Survival of plasmid-containing Bacillus subtilis released into mushroom compost
UNSPECIFIED (1998) Survival of plasmid-containing Bacillus subtilis released into mushroom compost. MICROBIAL ECOLOGY, 36 (1). pp. 51-59. ISSN 0095-3628Full text not available from this repository.
The survival of a plasmid-containing Bacillus subtilis released into mushroom compost was investigated. The indigenous Bacillus population of mushroom compost exhibited an antibioticresistance profile that was distinguished by almost complete absence of chloramphenicol resistance. Bacillus subtilis containing the chloramphenicol-resistance plasmid pC194 was released into mushroom compost microcosms and populations were monitored at different incubation temperatures. The organism colonized both sterile and untreated compost at 37 degrees C, and to a lesser extent at 50 degrees C, but was eliminated after 30 d at 65 degrees C. Although sporulation of the B. subtilis population occurred within compost, the population was maintained for up to 13 weeks at 50 degrees C, largely as vegetative cells. Experiments in which the B. subtilis host strain, without plasmid, was released demonstrated that plasmid carriage had no effect on the ability of the bacterium to colonize and survive in compost. Furthermore, the size and composition of the indigenous bacterial population was unaffected by the presence of the introduced B. subtilis strain. Virtually no loss of plasmid pC194 from the B. subtilis population in compost was observed, and experiments at low growth rates in chemostats confirmed the stability of this host/vector system in the absence of positive selection pressure.
|Item Type:||Journal Article|
|Subjects:||Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
|Journal or Publication Title:||MICROBIAL ECOLOGY|
|Number of Pages:||9|
|Page Range:||pp. 51-59|
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