UNSPECIFIED (1998) Production of toluene cis-diol by immobilized Pseudomonas putida UV4 cells in barium alginate beads. ENZYME AND MICROBIAL TECHNOLOGY, 22 (8). pp. 713-720. ISSN 0141-0229

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Abstract

In the present study, we have investigated the biotransformation of toluene to its cis-dihydrodiol (cis-diol) with immobilized Pseudomonas putida UV4 cells using different conditions of immobilization with a view to improving its production. The choice of alginate and its concentration for the immobilization of the cells were found to be the most important factors affecting the production of toluene cis-diol. The concentration of minerals and oxygen in the reaction medium and the methodology of substrate addition were investigated and the optimal conditions were defined. Once the optimal conditions for biotransformations and entrapment were determined a packed-bed and fluidized-bed reactor were evaluated for the biotransformation process. The results using air as the gas supply showed an increase in the total production from 0.15 mol cis-diol . g(-1) dry cell weight (dcw) in the packed-bed reactor to 0.28 mol cis-diol . g(-1) dcw in the fluidized-bed reactor When pure oxygen was used in place of air in the fluidized-bed reactor, a dramatic increase in total production up to a maximum of 6.1 mol cis-diol . g(-1 )dcw using a medium flow rate of 100 ml min(-1 )was achieved. Under optimal conditions, a maximum rate of production of 86.9 mmol cis-diol g(-1) dcw h(-1) was achieved for 48 h. This was seven times higher than the rate previously reported in the literature and for a much longer period of time; consequently, the overall production observed was more than 75 times higher than the values reported in the literature. (C) 1998 Elsevier Science Inc.

Item Type:	Journal Article
Subjects:	T Technology > TP Chemical technology
Journal or Publication Title:	ENZYME AND MICROBIAL TECHNOLOGY
Publisher:	ELSEVIER SCIENCE INC
ISSN:	0141-0229
Date:	June 1998
Volume:	22
Number:	8
Number of Pages:	8
Page Range:	pp. 713-720
Publication Status:	Published
URI:	http://wrap.warwick.ac.uk/id/eprint/15661

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