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Generating aptamers towards human sperm cells using massively parallel sequencing
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Gooch, James, Tungsirisurp, Sireethorn, Costanzo, Hayley, Napier, Richard and Frascione, Nunzianda (2021) Generating aptamers towards human sperm cells using massively parallel sequencing. Analytical and Bioanalytical Chemistry, 413 . pp. 5821-5834. doi:10.1007/s00216-021-03562-7 ISSN 1618-2642.
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Official URL: https://doi.org/10.1007/s00216-021-03562-7
Abstract
Determining the presence of sperm cells on an item or swab is often a crucial component of sexual offence investigation. However, traditional histological staining techniques used for the morphological identification of spermatozoa lack both specificity and sensitivity, making analysis a complex and time-consuming process. New methods for the detection of sperm cells based on aptamer recognition may be able to overcome these issues. In this work, we present the selection of ssDNA aptamers against human sperm cells using Cell-SELEX and massively parallel sequencing technologies. A total of 14 rounds of selection were performed following a modified Cell-SELEX protocol, which included additional steps for the isolation of spermatozoa from seminal fluid. Massively parallel sequencing using the Illumina Miseq platform was conducted on enriched aptamer pools to elucidate the structure of potential binders. A custom bioinformatics pipeline was also developed using Galaxy for the automated processing of sequencing datasets. This data revealed several promising aptamer candidates, which were shown to selectively bind sperm cells through both microscale thermophoresis and enzyme-linked oligonucleotide assays. These aptamers have the potential to increase the efficiency of sexual offence casework by facilitating sperm detection. [Abstract copyright: © 2021. The Author(s).]
| Item Type: | Journal Article | ||||||||
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| Subjects: | H Social Sciences > HV Social pathology. Social and public welfare Q Science > QL Zoology Q Science > QP Physiology R Medicine > R Medicine (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Library of Congress Subject Headings (LCSH): | Oligonucleotides , Spermatozoa, Nucleotide sequence -- Data processing, Biosensors , Forensic sciences | ||||||||
| Journal or Publication Title: | Analytical and Bioanalytical Chemistry | ||||||||
| Publisher: | Springer | ||||||||
| ISSN: | 1618-2642 | ||||||||
| Official Date: | September 2021 | ||||||||
| Dates: |
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| Volume: | 413 | ||||||||
| Page Range: | pp. 5821-5834 | ||||||||
| DOI: | 10.1007/s00216-021-03562-7 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 25 August 2021 | ||||||||
| Date of first compliant Open Access: | 25 August 2021 | ||||||||
| RIOXX Funder/Project Grant: |
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