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Identification and assessment of cardiolipin interactions with E. coli inner membrane proteins
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Corey, Robin A., Song, Wanling, Duncan, Anna L., Ansell, T. Bertie, Sansom, Mark S. P. and Stansfeld, Phillip J. (2021) Identification and assessment of cardiolipin interactions with E. coli inner membrane proteins. Science Advances, 7 (34). eabh2217. doi:10.1126/sciadv.abh2217
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WRAP-Identification-assessment-cardiolipin-interactions-E. coli-inner-membrane-proteins-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3123Kb) | Preview |
Official URL: https://doi.org/10.1126/sciadv.abh2217
Abstract
Integral membrane proteins are localized and/or regulated by lipids present in the surrounding bilayer. While bacteria have relatively simple membranes, there is ample evidence that many bacterial proteins bind to specific lipids, especially the anionic lipid cardiolipin. Here, we apply molecular dynamics simulations to assess lipid binding to 42 different Escherichia coli inner membrane proteins. Our data reveal an asymmetry between the membrane leaflets, with increased anionic lipid binding to the inner leaflet regions of the proteins, particularly for cardiolipin. From our simulations, we identify >700 independent cardiolipin binding sites, allowing us to identify the molecular basis of a prototypical cardiolipin binding site, which we validate against structures of bacterial proteins bound to cardiolipin. This allows us to construct a set of metrics for defining a high-affinity cardiolipin binding site on bacterial membrane proteins, paving the way for a heuristic approach to defining other protein-lipid interactions.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QP Physiology | |||||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cardiolipin, Membrane proteins, Lipids, Escherichia coli | |||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Science Advances | |||||||||||||||||||||||||||||||||
| Publisher: | American Association for the Advancement of Science | |||||||||||||||||||||||||||||||||
| ISSN: | 2375-2548 | |||||||||||||||||||||||||||||||||
| Official Date: | 20 August 2021 | |||||||||||||||||||||||||||||||||
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| Volume: | 7 | |||||||||||||||||||||||||||||||||
| Number: | 34 | |||||||||||||||||||||||||||||||||
| Article Number: | eabh2217 | |||||||||||||||||||||||||||||||||
| DOI: | 10.1126/sciadv.abh2217 | |||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||||||||||||||
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