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A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root
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Graeff, Moritz, Rana, Surbhi, Wendrich, Jos R., Dorier, Julien, Eekhout, Thomas, Aliaga Fandino, Ana Cecilia, Guex, Nicolas, Bassel, George, De Rybel, Bert and Hardtke, Christian S. (2021) A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root. Molecular Plant, 14 (12). pp. 1985-1999. doi:10.1016/j.molp.2021.07.021 ISSN 1674-2052.
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Official URL: http://dx.doi.org/10.1016/j.molp.2021.07.021
Abstract
The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but a simple consistent positive or negative impact on a basic cellular parameter was not identified. In this study, we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to characterize root meristems and mature root segments of brassinosteroid-blind mutants and wild type. The resultant datasets demonstrate that brassinosteroid signaling affects neither cell volume nor cell proliferation capacity. Instead, brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell expansion. Moreover, we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions, which are sufficient to restore growth vigor. Finally, single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QK Botany | |||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Arabidopsis, Brassinosteroids, Brassinosteroids -- Molecular aspects , Roots (Botany) -- Development , Meristems , Messenger RNA | |||||||||||||||||||||
| Journal or Publication Title: | Molecular Plant | |||||||||||||||||||||
| Publisher: | Oxford University Press | |||||||||||||||||||||
| ISSN: | 1674-2052 | |||||||||||||||||||||
| Official Date: | 6 December 2021 | |||||||||||||||||||||
| Dates: |
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| Volume: | 14 | |||||||||||||||||||||
| Number: | 12 | |||||||||||||||||||||
| Page Range: | pp. 1985-1999 | |||||||||||||||||||||
| DOI: | 10.1016/j.molp.2021.07.021 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||
| Date of first compliant deposit: | 15 September 2021 | |||||||||||||||||||||
| Date of first compliant Open Access: | 17 September 2021 | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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