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Multi‐modal adaptor‐clathrin contacts drive coated vesicle assembly

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Smith, Sarah Marie, Larocque, Gabrielle, Wood, Katherine M., Morris, Kyle L., Roseman, Alan M., Sessions, Richard B., Royle, Stephen J. and Smith, Corinne J. (2021) Multi‐modal adaptor‐clathrin contacts drive coated vesicle assembly. The EMBO Journal, 40 (19). e108795. doi:10.15252/embj.2021108795

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Official URL: http://dx.doi.org/10.15252/embj.2021108795

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Abstract

Clathrin-coated pits are formed by the recognition of membrane and cargo by the AP2 complex and the subsequent recruitment of clathrin triskelia. A role for AP2 in coated-pit assembly beyond initial clathrin recruitment has not been explored. Clathrin binds the β2 subunit of AP2, and several binding sites have been identified, but our structural knowledge of these interactions is incomplete and their functional importance during endocytosis is unclear. Here, we analysed the cryo-EM structure of clathrin cages assembled in the presence of β2 hinge-appendage (β2HA). We find that the β2-appendage binds in at least two positions in the cage, demonstrating that multi-modal binding is a fundamental property of clathrin-AP2 interactions. In one position, β2-appendage cross-links two adjacent terminal domains from different triskelia. Functional analysis of β2HA-clathrin interactions reveals that endocytosis requires two clathrin interaction sites: a clathrin-box motif on the hinge and the “sandwich site” on the appendage. We propose that β2-appendage binding to more than one triskelion is a key feature of the system and likely explains why assembly is driven by AP2.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
Divisions: Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Cell & Developmental Biology
Faculty of Medicine > Warwick Medical School > Biomedical Sciences
Faculty of Science > Life Sciences (2010- )
Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Endocytosis -- Research, Biological transport, Coated vesicles, Proteins
Journal or Publication Title: The EMBO Journal
Publisher: Nature Publishing Group
ISSN: 0261-4189
Official Date: 1 October 2021
Dates:
DateEvent
1 October 2021Published
6 September 2021Available
3 August 2021Accepted
Volume: 40
Number: 19
Article Number: e108795
DOI: 10.15252/embj.2021108795
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BB/N008391/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
UNSPECIFIEDLeverhulme Trusthttp://dx.doi.org/10.13039/501100000275
MR/N014294/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
MC_PC_17136[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
BB/M01228X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
MC_PC_17136[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

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