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Advances in engineering of bacteriophages for therapeutic applications
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Møller-Olsen, Christian, Dhanoa, Gurneet, Fehér, Tamás and Sagona, Antonia P. (2022) Advances in engineering of bacteriophages for therapeutic applications. In: Singh, Vijai, (ed.) New Frontiers and Applications of Synthetic Biology. London: Academic Press, pp. 215-229. ISBN 9780128244692
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Official URL: https://doi.org/10.1016/B978-0-12-824469-2.00001-4
Abstract
Synthetic biology has experienced an incredible advancement over the last decades with applications influencing every aspect of our daily lives. The capacity of synthetic biology has been instrumental in the life sciences and is now crossing into clinical settings. Driven by the accelerating evolution of antibiotic-resistant bacteria, the development of phages and phage-derived particles as antimicrobials is receiving renewed attention. In this chapter, we present the current tools and approaches for engineering synthetic bacteriophages, the therapeutic applications for which they are being used and highlight the potential risks and rewards. A wealth of tools and technologies have been and are being developed for the design and engineering of synthetic bacteriophages for therapeutic applications. While homologous recombination still underpins many recombineering approaches, modern technologies such as clustered regularly interspaced short palindromic repeat–Cas gene-editing and cell-free assembly are making an appearance, enabling a higher success rate. The therapeutic applications for which engineered phage and phage-derived particles used are numerous. These range from exploiting the antimicrobial properties of phage-expressed endolysins, or modifying a temperate phage to a virulent lifecycle, to exploiting phages as vectors transporting anticancer compounds or utilizing the high host-specificity for pathogen detection. In short, the opportunities are endless, and we discuss some of those here.
Item Type: | Book Item | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||
Publisher: | Academic Press | ||||||
Place of Publication: | London | ||||||
ISBN: | 9780128244692 | ||||||
Book Title: | New Frontiers and Applications of Synthetic Biology | ||||||
Editor: | Singh, Vijai | ||||||
Official Date: | 14 January 2022 | ||||||
Dates: |
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Page Range: | pp. 215-229 | ||||||
DOI: | 10.1016/B978-0-12-824469-2.00001-4 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Copyright Holders: | Copyright © 2022 Elsevier Inc. All rights reserved. | ||||||
Description: | Chapter 15 |
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Date of first compliant deposit: | 16 September 2021 | ||||||
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