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High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells
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Herdman, M., von Kügelgen, A., Kureisaite-Ciziene, D., Duman, R., El Omari, K., Garman, E. F., Kjaer, A., Kolokouris, D., Löwe, J., Wagner, A., Stansfeld, Phillip J. and Bharat, T. (2022) High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells. Structure, 30 (2). pp. 215-228. doi:10.1016/j.str.2021.10.012 ISSN 0969-2126.
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Official URL: https://doi.org/10.1016/j.str.2021.10.012
Abstract
Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Prokaryotes -- Physiology, Cytoplasm, Bacteria, Metal ions -- Research, Carrier proteins -- Research, Fluorescence microscopy, X-ray diffraction imaging | |||||||||||||||||||||||||||||||||||||||||||||
| Journal or Publication Title: | Structure | |||||||||||||||||||||||||||||||||||||||||||||
| Publisher: | Cell Press | |||||||||||||||||||||||||||||||||||||||||||||
| ISSN: | 0969-2126 | |||||||||||||||||||||||||||||||||||||||||||||
| Official Date: | 3 February 2022 | |||||||||||||||||||||||||||||||||||||||||||||
| Dates: |
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| Volume: | 30 | |||||||||||||||||||||||||||||||||||||||||||||
| Number: | 2 | |||||||||||||||||||||||||||||||||||||||||||||
| Page Range: | pp. 215-228 | |||||||||||||||||||||||||||||||||||||||||||||
| DOI: | 10.1016/j.str.2021.10.012 | |||||||||||||||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||||||||||||||
| Access rights to Published version: | Restricted or Subscription Access | |||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 27 September 2021 | |||||||||||||||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 7 February 2022 | |||||||||||||||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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