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Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA

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Hepp, Christof, Shiaelis, Nicolas, Robb, Nicole C., Vaughan, Alison, Matthews, Philippa C., Stoesser, Nicole, Crook, Derrick and Kapanidis, Achillefs N. (2021) Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA. Scientific Reports, 11 (1). 19579. doi:10.1038/s41598-021-98972-z ISSN 2045-2322.

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Official URL: https://doi.org/10.1038/s41598-021-98972-z

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Abstract

The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.

Item Type: Journal Article
Subjects: R Medicine > RA Public aspects of medicine
R Medicine > RB Pathology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
SWORD Depositor: Library Publications Router
Library of Congress Subject Headings (LCSH): COVID-19 (Disease), COVID-19 Pandemic, 2020-, Fluorescence in situ hybridization
Journal or Publication Title: Scientific Reports
Publisher: Nature Publishing Group UK
ISSN: 2045-2322
Official Date: 1 October 2021
Dates:
DateEvent
1 October 2021Published
9 September 2021Accepted
Volume: 11
Number: 1
Article Number: 19579
DOI: 10.1038/s41598-021-98972-z
Status: Peer Reviewed
Publication Status: Published
Reuse Statement (publisher, data, author rights): ** From Springer Nature via Jisc Publications Router ** History: received 12-05-2021; accepted 09-09-2021; registration 17-09-2021; pub-electronic 01-10-2021; online 01-10-2021; collection 12-2021. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 20 October 2021
Date of first compliant Open Access: 20 October 2021
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
DKR00620Royal Societyhttp://dx.doi.org/10.13039/501100000288
COVID-19 Research Response FundUniversity of Oxfordhttp://viaf.org/viaf/142129514
Studentship[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
BB/V001868/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
110164/Z/15/Z Wellcome Trusthttp://dx.doi.org/10.13039/100010269
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