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Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA
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Hepp, Christof, Shiaelis, Nicolas, Robb, Nicole C., Vaughan, Alison, Matthews, Philippa C., Stoesser, Nicole, Crook, Derrick and Kapanidis, Achillefs N. (2021) Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA. Scientific Reports, 11 (1). 19579. doi:10.1038/s41598-021-98972-z
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Official URL: https://doi.org/10.1038/s41598-021-98972-z
Abstract
The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic exacerbates the need for rapid, affordable and sensitive methods for virus detection, identification and quantification; however, existing methods for detecting virus particles in biological samples usually depend on multistep protocols that take considerable time to yield a result. Here, we introduce a rapid fluorescence in situ hybridization (FISH) protocol capable of detecting influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically and quantitatively in approximately 20 min, in virus cultures, combined nasal and throat swabs with added virus and likely patient samples without previous purification. This fast and facile workflow can be adapted both as a lab technique and a future diagnostic tool in enveloped viruses with an accessible genome.
| Item Type: | Journal Article | ||||||||||||||||||
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| Subjects: | R Medicine > RA Public aspects of medicine R Medicine > RB Pathology |
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| Divisions: | Faculty of Medicine > Warwick Medical School | ||||||||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | COVID-19 (Disease), COVID-19 Pandemic, 2020-, Fluorescence in situ hybridization | ||||||||||||||||||
| Journal or Publication Title: | Scientific Reports | ||||||||||||||||||
| Publisher: | Nature Publishing Group UK | ||||||||||||||||||
| ISSN: | 2045-2322 | ||||||||||||||||||
| Official Date: | 1 October 2021 | ||||||||||||||||||
| Dates: |
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| Volume: | 11 | ||||||||||||||||||
| Number: | 1 | ||||||||||||||||||
| Article Number: | 19579 | ||||||||||||||||||
| DOI: | 10.1038/s41598-021-98972-z | ||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||
| Publisher Statement: | ** From Springer Nature via Jisc Publications Router ** History: received 12-05-2021; accepted 09-09-2021; registration 17-09-2021; pub-electronic 01-10-2021; online 01-10-2021; collection 12-2021. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ | ||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||
| RIOXX Funder/Project Grant: |
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