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Novel point-of-care diagnostic method for neonatal encephalopathy using purine nucleosides
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Beamer, Edward, O'Dea, Mary Isabel, Garvey, Aisling A., Smith, Jonathon, Menéndez-Méndez, Aida, Kelly, Lynne, Pavel, Andreea, Quinlan, Sean, Alves, Mariana, Jimenez-Mateos, Eva M., Tian, Faming, Dempsey, Eugene, Dale, Nicholas, Murray, Deirdre M., Boylan, Geraldine B., Molloy, Eleanor J. and Engel, Tobias (2021) Novel point-of-care diagnostic method for neonatal encephalopathy using purine nucleosides. Frontiers in Molecular Neuroscience, 14 . 732199. doi:10.3389/fnmol.2021.732199 ISSN 1662-5099.
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Official URL: https://doi.org/10.3389/fnmol.2021.732199
Abstract
Evidence suggests that earlier diagnosis and initiation of treatment immediately after birth is critical for improved neurodevelopmental outcomes following neonatal encephalopathy (NE). Current diagnostic tests are, however, mainly restricted to clinical diagnosis with no molecular tests available. Purines including adenosine are released during brain injury such as hypoxia and are also present in biofluids. Whether blood purine changes can be used to diagnose NE has not been investigated to date. Blood purines were measured in a mouse model of neonatal hypoxia and infants with NE using a novel point-of-care diagnostic technology (SMARTChip) based on the summated electrochemical detection of adenosine and adenosine metabolites in the blood. Blood purine concentrations were ∼2-3-fold elevated following hypoxia in mice [2.77 ± 0.48 μM (Control) vs. 7.57 ± 1.41 μM (post-hypoxia), = 0.029]. Data in infants with NE had a 2-3-fold elevation when compared to healthy controls [1.63 ± 0.47 μM (Control, = 5) vs. 4.87 ± 0.92 μM (NE, = 21), = 0.0155]. ROC curve analysis demonstrates a high sensitivity (81%) and specificity (80%) for our approach to identify infants with NE. Moreover, blood purine concentrations were higher in infants with NE and seizures [8.13 ± 3.23 μM (with seizures, = 5) vs. 3.86 ± 0.56 μM (without seizures, = 16), = 0.044]. Our data provides the proof-of-concept that measurement of blood purine concentrations via SMARTChip technology may offer a low-volume bedside test to support a rapid diagnosis of NE. [Abstract copyright: Copyright © 2021 Beamer, O’Dea, Garvey, Smith, Menéndez-Méndez, Kelly, Pavel, Quinlan, Alves, Jimenez-Mateos, Tian, Dempsey, Dale, Murray, Boylan, Molloy and Engel.]
Item Type: | Journal Article | |||||||||||||||||||||||||||
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Subjects: | Q Science > QH Natural history Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RJ Pediatrics |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||
SWORD Depositor: | Library Publications Router | |||||||||||||||||||||||||||
Library of Congress Subject Headings (LCSH): | Hypoxic-ischemic encephalopathy, Biochemical markers, Purines, Infantile spasms | |||||||||||||||||||||||||||
Journal or Publication Title: | Frontiers in Molecular Neuroscience | |||||||||||||||||||||||||||
Publisher: | Frontiers Research Foundation | |||||||||||||||||||||||||||
ISSN: | 1662-5099 | |||||||||||||||||||||||||||
Official Date: | 9 September 2021 | |||||||||||||||||||||||||||
Dates: |
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Volume: | 14 | |||||||||||||||||||||||||||
Article Number: | 732199 | |||||||||||||||||||||||||||
DOI: | 10.3389/fnmol.2021.732199 | |||||||||||||||||||||||||||
Status: | Peer Reviewed | |||||||||||||||||||||||||||
Publication Status: | Published | |||||||||||||||||||||||||||
Reuse Statement (publisher, data, author rights): | ** From PubMed via Jisc Publications Router ** History: received 28-06-2021; accepted 19-08-2021. | |||||||||||||||||||||||||||
Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||
Date of first compliant deposit: | 12 October 2021 | |||||||||||||||||||||||||||
Date of first compliant Open Access: | 13 October 2021 | |||||||||||||||||||||||||||
RIOXX Funder/Project Grant: |
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