Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides
UNSPECIFIED (1998) Sec/SRP-independent insertion of two thylakoid membrane proteins bearing cleavable signal peptides. FEBS LETTERS, 424 (1-2). pp. 105-108. ISSN 0014-5793Full text not available from this repository.
Two imported thylakoid membrane proteins, PSII-X and PSII-W, are synthesised with cleavable N-terminal signal peptides that closely resemble those of Sec-dependent lumenal proteins. In this report we have reconstituted the insertion of pre-PSII-X and pre-PSII-W into isolated thylakoids. We show that insertion does not require either nucleoside triphosphates or stromal extracts, both of which are required for Sec- and signal recognition particle (SRP)-dependent targeting mechanisms. Insertion is furthermore unaffected by protease treatments that destroy the known protein translocation apparatus in the thylakoid membrane. We conclude that these membrane proteins are inserted by an unusual Sec/SRP-independent mechanism that probably resembles that used by CFoII, and me discuss possible parallels with the biogenesis of phage M13 procoat. (C) 1998 Federation of European Biochemical Societies.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Journal or Publication Title:||FEBS LETTERS|
|Publisher:||ELSEVIER SCIENCE BV|
|Date:||6 March 1998|
|Number of Pages:||4|
|Page Range:||pp. 105-108|
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