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Expression of putative effectors of different Xylella fastidiosa strains triggers cell death‐like responses in various Nicotiana model plants
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Sertedakis, Matthaios, Kotsaridis, Konstantinos, Tsakiri, Dimitra, Mermigka, Glykeria, Dominguez‐Ferreras, Ana, Ntoukakis, Vardis and Sarris, Panagiotis F. (2022) Expression of putative effectors of different Xylella fastidiosa strains triggers cell death‐like responses in various Nicotiana model plants. Molecular Plant Pathology, 23 (1). pp. 148-156. doi:10.1111/mpp.13147 ISSN 1364-3703.
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WRAP-Expression-putative-effectors-different-Xylella-fastidiosa-strains-triggers-cell-death‐Nicotiana-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons: Attribution-Noncommercial 4.0. Download (1016Kb) | Preview |
Official URL: https://doi.org/10.1111/mpp.13147
Abstract
The wide host range of Xylella fastidiosa (Xf) indicates the existence of yet uncharacterized virulence mechanisms that help pathogens to overcome host defences. Various bioinformatics tools combined with prediction of the functions of putative virulence proteins are valuable approaches to study microbial pathogenicity. We collected a number of putative effectors from three Xf strains belonging to different subspecies: Temecula-1 (subsp. fastidiosa), CoDiRO (subsp. pauca), and Ann-1 (subsp. sandyi). We designed an in planta Agrobacterium-based expression system that drives the expressed proteins to the cell apoplast, in order to investigate their ability to activate defence in Nicotiana model plants. Multiple Xf proteins differentially elicited cell death-like phenotypes in different Nicotiana species. These proteins are members of different enzymatic groups: (a) hydrolases/hydrolase inhibitors, (b) serine proteases, and (c) metal transferases. We also classified the Xf proteins according to their sequential and structural similarities via the I-TASSER online tool. Interestingly, we identified similar proteins that were able to differentially elicit cell death in different cultivars of the same species. Our findings provide a basis for further studies on the mechanisms that underlie both defence activation in Xf resistant hosts and pathogen adaptation in susceptible hosts.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QK Botany | |||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Plant diseases -- Research, Trees -- Diseases and pests, Pathogenic bacteria, Xylella fastidiosa, Bacterial diseases of plants, Cell death -- Research, Plant physiology -- Research | |||||||||
| Journal or Publication Title: | Molecular Plant Pathology | |||||||||
| Publisher: | Wiley | |||||||||
| ISSN: | 1364-3703 | |||||||||
| Official Date: | January 2022 | |||||||||
| Dates: |
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| Volume: | 23 | |||||||||
| Number: | 1 | |||||||||
| Page Range: | pp. 148-156 | |||||||||
| DOI: | 10.1111/mpp.13147 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Copyright Holders: | © 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd. | |||||||||
| Date of first compliant deposit: | 25 October 2021 | |||||||||
| Date of first compliant Open Access: | 26 October 2021 | |||||||||
| RIOXX Funder/Project Grant: |
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