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The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs
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UNSPECIFIED (1997) The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs. FEMS MICROBIOLOGY LETTERS, 156 (2). pp. 205-210. ISSN 0378-1097
Full text not available from this repository.Abstract
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones retrieved directly from environmental DNA samples revealed that they form a distinct cluster within representative PmoA sequences from type II methanotrophs and may originate from a novel group of acidophilic methanotrophs. The study also demonstrated the utility of the pmoA gene as a phylogenetic marker for identifying methanotroph-specific DNA sequences in the environment.
| Item Type: | Journal Article |
|---|---|
| Subjects: | Q Science > QR Microbiology |
| Journal or Publication Title: | FEMS MICROBIOLOGY LETTERS |
| Publisher: | ELSEVIER SCIENCE BV |
| ISSN: | 0378-1097 |
| Date: | 15 November 1997 |
| Volume: | 156 |
| Number: | 2 |
| Number of Pages: | 6 |
| Page Range: | pp. 205-210 |
| Publication Status: | Published |
| URI: | http://wrap.warwick.ac.uk/id/eprint/15965 |
Data sourced from Thomson Reuters' Web of Knowledge
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