The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs
UNSPECIFIED (1997) The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs. FEMS MICROBIOLOGY LETTERS, 156 (2). pp. 205-210. ISSN 0378-1097Full text not available from this repository.
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source. The resulting 525 bp PCR products were cloned and a representative number of clones were sequenced. Phylogenetic analysis of the derived amino acid sequences of the pmoA clones retrieved directly from environmental DNA samples revealed that they form a distinct cluster within representative PmoA sequences from type II methanotrophs and may originate from a novel group of acidophilic methanotrophs. The study also demonstrated the utility of the pmoA gene as a phylogenetic marker for identifying methanotroph-specific DNA sequences in the environment.
|Item Type:||Journal Article|
|Subjects:||Q Science > QR Microbiology|
|Journal or Publication Title:||FEMS MICROBIOLOGY LETTERS|
|Publisher:||ELSEVIER SCIENCE BV|
|Date:||15 November 1997|
|Number of Pages:||6|
|Page Range:||pp. 205-210|
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