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In vitro elucidation of the crucial but complex oxidative tailoring steps in rufomycin biosynthesis enables one pot conversion of rufomycin B to rufomycin C
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Perez Ortiz, Gustavo, Sidda, John D., de los Santos, Emmanuel L. C., Hubert, Catherine B. and Barry, Sarah M. (2021) In vitro elucidation of the crucial but complex oxidative tailoring steps in rufomycin biosynthesis enables one pot conversion of rufomycin B to rufomycin C. Chemical Communications, 57 (89). pp. 11795-11798. doi:10.1039/d1cc04794a
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WRAP-In-vitro-elucidation-crucial-complex-oxidative-tailoring-steps-rufomycin-biosynthesis-enables-one-pot-conversion-rufomycin-B-rufomycin-C-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution. Download (2773Kb) | Preview |
Official URL: https://doi.org/10.1039/d1cc04794a
Abstract
The antimycobacterial peptides, rufomycins, have their antibiotic activity conferred by oxidative tailoring of the cyclic peptide. Here we elucidate the roles of cytochrome P450s RufS and RufM in regioselective epoxidation and alkyl oxidation respectively and demonstrate how RufM and RufS create a complex product profile dependent on redox partner availability. Finally, we report the in vitro one pot conversion of rufomycin B to rufomycin C.
| Item Type: | Journal Article | ||||||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||
| SWORD Depositor: | Library Publications Router | ||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Peptides , Cyclic peptides , Cytochrome P-450, Epoxy compounds, Biosynthesis | ||||||||||||||||||||||||
| Journal or Publication Title: | Chemical Communications | ||||||||||||||||||||||||
| Publisher: | Royal Society of Chemistry | ||||||||||||||||||||||||
| ISSN: | 1359-7345 | ||||||||||||||||||||||||
| Official Date: | 2021 | ||||||||||||||||||||||||
| Dates: |
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| Volume: | 57 | ||||||||||||||||||||||||
| Number: | 89 | ||||||||||||||||||||||||
| Page Range: | pp. 11795-11798 | ||||||||||||||||||||||||
| DOI: | 10.1039/d1cc04794a | ||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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