Mandala, Samuel Husin Surya, Liu, Tai-Jan, Chen, Chiung-Mei, Liu, Kuo-Kang, Januar, Mochamad, Chang, Ying-Feng, Lai, Chao-Sung, Chang, Kuo-Hsuan and Liu, Kou-Chen (2021) Enhanced plasmonic biosensor utilizing paired antibody and label-free Fe3O4 nanoparticles for highly sensitive and selective detection of Parkinson’s α-synuclein in serum. Biosensors, 11 (10). 402. doi:10.3390/bios11100402 ISSN 2079-6374.

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Abstract

Parkinson’s disease (PD) is an acute and progressive neurodegenerative disorder, and diagnosis of the disease at its earliest stage is of paramount importance to improve the life expectancy of patients. α-Synuclein (α-syn) is a potential biomarker for the early diagnosis of PD, and there is a great need to develop a biosensing platform that precisely detects α-syn in human body fluids. Herein, we developed a surface plasmon resonance (SPR) biosensor based on the label-free iron oxide nanoparticles (Fe3O4 NPs) and paired antibody for the highly sensitive and selective detection of α-syn in serum samples. The sensitivity of the SPR platform is enhanced significantly by directly depositing Fe3O4 NPs on the Au surface at a high density to increase the decay length of the evanescent field on the Au film. Moreover, the utilization of rabbit-type monoclonal antibody (α-syn-RmAb) immobilized on Au films allows the SPR platform to have a high affinity-selectivity binding performance compared to mouse-type monoclonal antibodies as a common bioreceptor for capturing α-syn molecules. As a result, the current platform has a detection limit of 5.6 fg/mL, which is 20,000-fold lower than that of commercial ELISA. The improved sensor chip can also be easily regenerated to repeat the α-syn measurement with the same sensitivity. Furthermore, the SPR sensor was applied to the direct analysis of α-syn in serum samples. By using a format of paired α-syn-RmAb, the SPR sensor provides a recovery rate in the range from 94.5% to 104.3% to detect the α-syn in diluted serum samples precisely. This work demonstrates a highly sensitive and selective quantification approach to detect α-syn in human biofluids and paves the way for the future development in the early diagnosis of PD.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry Q Science > QP Physiology R Medicine > R Medicine (General) R Medicine > RC Internal medicine R Medicine > RS Pharmacy and materia medica
Divisions:	Faculty of Science, Engineering and Medicine > Engineering > Engineering
Library of Congress Subject Headings (LCSH):	Synucleins , Parkinson's disease, Parkinson's disease -- Prognosis, Parkinson's disease -- Prevention , Surface plasmon resonance , Biosensors , Ferric oxide, Nanoparticles -- Therapeutic use, Serum
Journal or Publication Title:	Biosensors
Publisher:	M D P I AG
ISSN:	2079-6374
Official Date:	18 October 2021
Dates:	Date Event 18 October 2021 Published 14 October 2021 Accepted 19 September 2021 Submitted
Volume:	11
Number:	10
Article Number:	402
DOI:	10.3390/bios11100402
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access (Creative Commons)
Date of first compliant deposit:	8 November 2021
Date of first compliant Open Access:	9 November 2021
RIOXX Funder/Project Grant:	Project/Grant ID RIOXX Funder Name Funder ID 109-2221-E-182-063-MY3 Ministry of Science and Technology, Taiwan http://dx.doi.org/10.13039/501100004663 109-2221-E-182-021-MY3 Ministry of Science and Technology, Taiwan http://dx.doi.org/10.13039/501100004663 CMRPD2G0152 Chang Gung Memorial Hospital http://dx.doi.org/10.13039/100012553

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