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An in vitro model for the cultivation of polymicrobial biofilms under continuous-flow conditions [version 1; peer review: 2 approved]
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O'Brien, Thomas James, Hassan, Marwa Mohsen, Harrison, Freya and Welch, Martin (2021) An in vitro model for the cultivation of polymicrobial biofilms under continuous-flow conditions [version 1; peer review: 2 approved]. F1000Research, 10 . p. 801. doi:10.12688/f1000research.55140.1 ISSN 2046-1402.
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WRAP-An-in-vitro-model-cultivation-polymicrobial-biofilms-continuous-flow-conditions-v1-2-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3453Kb) | Preview |
Official URL: http://dx.doi.org/10.12688/f1000research.55140.1
Abstract
The airways of people with cystic fibrosis (CF) are often chronically colonised with a diverse array of bacterial and fungal species. However, little is known about the relative partitioning of species between the planktonic and biofilm modes of growth in the airways. Existing in vivo and in vitro models of CF airway infection are ill-suited for the long-term recapitulation of mixed microbial communities. Here we describe a simple, in vitro continuous-flow model for the cultivation of polymicrobial biofilms and planktonic cultures on different substrata. Our data provide evidence for inter-species antagonism and synergism in biofilm ecology. We further show that the type of substratum on which the biofilms grow has a profound influence on their species composition. This happens without any major alteration in the composition of the surrounding steady-state planktonic community. Our experimentally-tractable model enables the systematic study of planktonic and biofilm communities under conditions that are nutritionally reminiscent of the CF airway microenvironment, something not possible using any existing in vivo models of CF airway infection.
| Item Type: | Journal Article | |||||||||||||||
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| Subjects: | R Medicine > R Medicine (General) R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||
| Library of Congress Subject Headings (LCSH): | Cystic fibrosis , Cystic fibrosis -- Treatment, Polymers in medicine , Biofilms | |||||||||||||||
| Journal or Publication Title: | F1000Research | |||||||||||||||
| Publisher: | F1000 Research Ltd | |||||||||||||||
| ISSN: | 2046-1402 | |||||||||||||||
| Official Date: | 13 August 2021 | |||||||||||||||
| Dates: |
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| Volume: | 10 | |||||||||||||||
| Page Range: | p. 801 | |||||||||||||||
| DOI: | 10.12688/f1000research.55140.1 | |||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||
| Publication Status: | Published | |||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||
| Date of first compliant deposit: | 12 November 2021 | |||||||||||||||
| Date of first compliant Open Access: | 15 November 2021 | |||||||||||||||
| RIOXX Funder/Project Grant: |
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