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An in vitro model for the cultivation of polymicrobial biofilms under continuous-flow conditions [version 1; peer review: 2 approved]

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O'Brien, Thomas James, Hassan, Marwa Mohsen, Harrison, Freya and Welch, Martin (2021) An in vitro model for the cultivation of polymicrobial biofilms under continuous-flow conditions [version 1; peer review: 2 approved]. F1000Research, 10 . p. 801. doi:10.12688/f1000research.55140.1

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Official URL: http://dx.doi.org/10.12688/f1000research.55140.1

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Abstract

The airways of people with cystic fibrosis (CF) are often chronically colonised with a diverse array of bacterial and fungal species. However, little is known about the relative partitioning of species between the planktonic and biofilm modes of growth in the airways. Existing in vivo and in vitro models of CF airway infection are ill-suited for the long-term recapitulation of mixed microbial communities. Here we describe a simple, in vitro continuous-flow model for the cultivation of polymicrobial biofilms and planktonic cultures on different substrata. Our data provide evidence for inter-species antagonism and synergism in biofilm ecology. We further show that the type of substratum on which the biofilms grow has a profound influence on their species composition. This happens without any major alteration in the composition of the surrounding steady-state planktonic community. Our experimentally-tractable model enables the systematic study of planktonic and biofilm communities under conditions that are nutritionally reminiscent of the CF airway microenvironment, something not possible using any existing in vivo models of CF airway infection.

Item Type: Journal Article
Subjects: R Medicine > R Medicine (General)
R Medicine > RC Internal medicine
Divisions: Faculty of Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Cystic fibrosis , Cystic fibrosis -- Treatment, Polymers in medicine , Biofilms
Journal or Publication Title: F1000Research
Publisher: F1000 Research Ltd
ISSN: 2046-1402
Official Date: 13 August 2021
Dates:
DateEvent
13 August 2021Published
13 August 2021Accepted
Volume: 10
Page Range: p. 801
DOI: 10.12688/f1000research.55140.1
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
NC/P001564/1National Centre for the Replacement Refinement and Reduction of Animals in Researchhttp://dx.doi.org/10.13039/501100010757
UNSPECIFIEDCystic Fibrosis Trusthttp://dx.doi.org/10.13039/501100000292
UNSPECIFIEDBritish Lung Foundationhttp://dx.doi.org/10.13039/501100000351
MR/R001898/1[MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265

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