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D-alanine metabolism via D-Ala aminotransferase by marine Gammaproteobacteria, Pseudoalteromonas sp. CF6-2
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Yu, Yang, Yang, Jie, Teng, Zhao-Jie, Zheng, Li-Yuan, Sheng, Qi, Li, Ping-Yi, Fu, Hui-Hui, Li, Chun-Yang, Chen, Yin, Zhang, Yu-Zhong, Ding, Jun-Mei and Chen, Xiu-Lan (2022) D-alanine metabolism via D-Ala aminotransferase by marine Gammaproteobacteria, Pseudoalteromonas sp. CF6-2. Applied and Environmental Microbiology, 88 (3). doi:10.1128/aem.02219-21 ISSN 1098-5336.
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Official URL: https://doi.org/10.1128/aem.02219-21
Abstract
As the most abundant d-amino acid (DAA) in the ocean, d-alanine (d-Ala) is a key component of peptidoglycan in the bacterial cell wall. However, the underlying mechanisms of bacterial metabolization of d-Ala through the microbial food web remain largely unknown. In this study, the metabolism of d-Ala by marine bacterium Pseudoalteromonas sp. strain CF6-2 was investigated. Based on genomic, transcriptional, and biochemical analyses combined with gene knockout, d-Ala aminotransferase was found to be indispensable for the catabolism of d-Ala in strain CF6-2. Investigation on other marine bacteria also showed that d-Ala aminotransferase gene is a reliable indicator for their ability to utilize d-Ala. Bioinformatic investigation revealed that d-Ala aminotransferase sequences are prevalent in genomes of marine bacteria and metagenomes, especially in seawater samples, and Gammaproteobacteria represents the predominant group containing d-Ala aminotransferase. Thus, Gammaproteobacteria is likely the dominant group to utilize d-Ala via d-Ala aminotransferase to drive the recycling and mineralization of d-Ala in the ocean.
IMPORTANCE As the most abundant d-amino acid in the ocean, d-Ala is a component of the marine DON (dissolved organic nitrogen) pool. However, the underlying mechanism of bacterial metabolization of d-Ala to drive the recycling and mineralization of d-Ala in the ocean is still largely unknown. The results in this study showed that d-Ala aminotransferase is specific and indispensable for d-Ala catabolism in marine bacteria and that marine bacteria containing d-Ala aminotransferase genes are predominantly Gammaproteobacteria widely distributed in global oceans. This study reveals marine d-Ala-utilizing bacteria and the mechanism of their metabolization of d-Ala. The results shed light on the mechanisms of recycling and mineralization of d-Ala driven by bacteria in the ocean, which are helpful in understanding oceanic microbial-mediated nitrogen cycle.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Journal or Publication Title: | Applied and Environmental Microbiology | ||||||||
Publisher: | American Society for Microbiology | ||||||||
ISSN: | 1098-5336 | ||||||||
Official Date: | 8 February 2022 | ||||||||
Dates: |
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Volume: | 88 | ||||||||
Number: | 3 | ||||||||
DOI: | 10.1128/aem.02219-21 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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