The Library
Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe
Tools
Tools
Tools
(2022) Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe. Nucleic Acids Research, 50 (1). pp. 350-367. gkab1249. doi:10.1093/nar/gkab1249
|
PDF
WRAP-Genome-wide-chromosomal-association-Upf1-schizosaccharomyces-2021.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3571Kb) | Preview |
|
![[img]](http://wrap.warwick.ac.uk/style/images/fileicons/application_pdf.png) |
PDF
lf-031221-wrap--sandip_de.pdf - Accepted Version Embargoed item. Restricted access to Repository staff only - Requires a PDF viewer. Download (4Mb) |
Official URL: https://doi.org/10.1093/nar/gkab1249
Abstract
Although the RNA helicase Upf1 has hitherto been examined mostly in relation to its cytoplasmic role in nonsense mediated mRNA decay (NMD), here we report high-throughput ChIP data indicating genome-wide association of Upf1 with active genes in Schizosaccharomyces pombe. This association is RNase sensitive, correlates with Pol II transcription and mRNA expression levels. Changes in Pol II occupancy were detected in a Upf1 deficient (upf1∆) strain, prevalently at genes showing a high Upf1 relative to Pol II association in wild-type. Additionally, an increased Ser2 Pol II signal was detected at all highly transcribed genes examined by ChIP-qPCR. Furthermore, upf1cells are hypersensitive to the transcription elongation inhibitor 6-azauracil. A significant proportion of the genes associated with Upf1 in wild-type conditions are also mis-regulated in upf1. These data envisage that by operating on the nascent transcript, Upf1 might influence Pol II phosphorylation and transcription.
| Item Type: | Journal Article | ||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Subjects: | Q Science > QH Natural history Q Science > QK Botany Q Science > QP Physiology |
||||||||||||||||||||||||||||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Schizosaccharomyces pombe -- Research, DNA helicases, Genomes, Messenger RNA, Eukaryotic cells -- Genetics | ||||||||||||||||||||||||||||||
| Journal or Publication Title: | Nucleic Acids Research | ||||||||||||||||||||||||||||||
| Publisher: | Oxford University Press | ||||||||||||||||||||||||||||||
| ISSN: | 0305-1048 | ||||||||||||||||||||||||||||||
| Official Date: | 11 January 2022 | ||||||||||||||||||||||||||||||
| Dates: |
|
||||||||||||||||||||||||||||||
| Volume: | 50 | ||||||||||||||||||||||||||||||
| Number: | 1 | ||||||||||||||||||||||||||||||
| Page Range: | pp. 350-367 | ||||||||||||||||||||||||||||||
| Article Number: | gkab1249 | ||||||||||||||||||||||||||||||
| DOI: | 10.1093/nar/gkab1249 | ||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | ||||||||||||||||||||||||||||||
| Publication Status: | Published | ||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | ||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
|
||||||||||||||||||||||||||||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
