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An integrative epi-transcriptomic approach identifies the human cartilage chitinase 3-like protein 2 (CHI3L2) as a potential mediator of B12 deficiency in adipocytes
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Ogunkolade, B. William, Adaikalakoteswari, Antonysunil, Cardoso, Shirleny Romualdo, Lowe, Rob, Patel, Nisha, Rakyan, Vardhman, Finer, Sarah, Wabitsch, Martin, Saravanan, Ponnusamy, Tripathi, Gyanendra, Bochukova, Elena and Hitman, Graham A. (2022) An integrative epi-transcriptomic approach identifies the human cartilage chitinase 3-like protein 2 (CHI3L2) as a potential mediator of B12 deficiency in adipocytes. Epigenetics, 17 (10). pp. 1219-1233. doi:10.1080/15592294.2021.2003043 ISSN 1559-2308.
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WRAP-integrative-epi-transcriptomic-approach-identifies-human-cartilage-chitinase-protein-deficiency-adipocytes-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution Non-commercial No Derivatives 4.0. Download (2422Kb) | Preview |
Official URL: https://doi.org/10.1080/15592294.2021.2003043
Abstract
Vitamin B12 has multiple biochemical functions including in the one-carbon cycle generating a methyl group for DNA methylation, and metabolism of fatty acids and amino acids to generate energy via the citric acid cycle. The aim of our study was to use a combined epigenomic and transcriptomic approach to identify novel genes mediating the effect of B12 on adipogenesis.
Human pre-adipocytes (CHUB-S7) were treated with a range of B12 (0–500 nM) concentrations from the day of cell seeding until harvesting in discovery and validation experiments prior to genome-wide methylation analysis using the Illumina HumanMethylation 450Beadchip. For transcriptomic analysis, RNA-seq libraries were run on the Illumina HiSeq 2500. To further investigate the expression of any genes on human adipogenesis, a second human preadipocyte strain was studied (SGBS) by real-time quantitative PCR (qRT-PCR).
A combined epigenetic and transcriptomic approach in differentiated human pre-adipocyte cell line, CHUB-S7, identified that the Human cartilage chitinase 3-like protein 2 (CHI3L2) gene was hypo-methylated and had increased expression in low B12 conditions. Furthermore, there was an approximately 1000-fold increase in CHI3L2 expression in the early days of adipocyte differentiation, which paralleled an increase of lipid droplets in differentiated SGBS cells and an increased expression level of markers of mature adipocytes.
In summary, we have identified a potential role of the human cartilage chitinase 3-like protein 2 (CHI3L2) in adipocyte function in the presence of low B12 levels.
| Item Type: | Journal Article | |||||||||
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| Subjects: | Q Science > QP Physiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | |||||||||
| SWORD Depositor: | Library Publications Router | |||||||||
| Library of Congress Subject Headings (LCSH): | Vitamin B12 deficiency -- Research, Vitamin B12 -- Physiological aspects, Fat cells, Chitinase | |||||||||
| Journal or Publication Title: | Epigenetics | |||||||||
| Publisher: | Informa UK Limited | |||||||||
| ISSN: | 1559-2308 | |||||||||
| Official Date: | 2022 | |||||||||
| Dates: |
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| Volume: | 17 | |||||||||
| Number: | 10 | |||||||||
| Page Range: | pp. 1219-1233 | |||||||||
| DOI: | 10.1080/15592294.2021.2003043 | |||||||||
| Status: | Peer Reviewed | |||||||||
| Publication Status: | Published | |||||||||
| Reuse Statement (publisher, data, author rights): | ** From Crossref journal articles via Jisc Publications Router ** History: epub 25-11-2021; issued 25-11-2021. | |||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||
| Date of first compliant deposit: | 26 January 2022 | |||||||||
| Date of first compliant Open Access: | 26 January 2022 | |||||||||
| RIOXX Funder/Project Grant: |
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