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Resurrecting Golgi proteins to grasp Golgi ribbon formation and self-association under stress
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Mendes, Luis F. S., Batista, Mariana R. B., Kava, Emanuel, Bleicher, Lucas, Micheletto, Mariana C. and Costa-Filho, Antonio J. (2022) Resurrecting Golgi proteins to grasp Golgi ribbon formation and self-association under stress. International Journal of Biological Macromolecules, 194 . pp. 264-275. doi:10.1016/j.ijbiomac.2021.11.173 ISSN 0141-8130.
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Official URL: https://doi.org/10.1016/j.ijbiomac.2021.11.173
Abstract
The Golgi complex is an essential organelle of the eukaryotic exocytic pathway. A subfamily of Golgi matrix proteins, called GRASPs, is central in stress-induced unconventional secretion, Golgi dynamics during mitosis/apoptosis, and Golgi ribbon formation. The Golgi ribbon is vertebrate-specific and correlates with the appearance of two GRASP paralogues and two Golgins (GM130/Golgin45), which form specific GRASP-Golgin pairs. The molecular details of their appearance only in Metazoans are unknown. Moreover, despite new functionalities supported by GRASP paralogy, little is known about their structural and evolutionary differences. Here, we used ancestor sequence reconstruction and biophysical/biochemical approaches to assess the evolution of GRASPs structure/dynamics, fibrillation, and how they started anchoring their Golgin partners. Our data showed that a GRASP ancestor anchored Golgins before gorasp gene duplication in Metazoans. After gene duplication, variations within the GRASP binding pocket determined which paralogue would recruit which Golgin. These interactions are responsible for their specific Golgi location and Golgi ribbon appearance. We also suggest that GRASPs have a long-standing capacity to form supramolecular structures, affecting their participation in stress-induced processes.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
SWORD Depositor: | Library Publications Router | ||||||||
Journal or Publication Title: | International Journal of Biological Macromolecules | ||||||||
Publisher: | Elsevier | ||||||||
ISSN: | 0141-8130 | ||||||||
Official Date: | 1 January 2022 | ||||||||
Dates: |
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Volume: | 194 | ||||||||
Page Range: | pp. 264-275 | ||||||||
DOI: | 10.1016/j.ijbiomac.2021.11.173 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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