Teahan, James (2019) Development of techniques for mapping analytes and properties at interfaces. PhD thesis, University of Warwick.

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Abstract

Scanning ion conductance microscopy (SICM) is a powerful and non-invasive tool which allows substrates to be probed at the nanoscale. Recently developed SICM functional mapping methodologies are capable of providing detailed information about interfacial properties and processes. Interaction of these techniques with complex active surfaces are not fully understood and much of the previous work in this area has been performed on ideal or model substrates. This thesis aims to extend the use of SICM functional mapping towards analysis of complex substrates and realworld samples.

Through application of potential control programs and extensive finite element method (FEM) simulation, the interactions between the SICM probe and substrate can be elucidated to explain the ion current response. SICM delivery methodologies are applied to examine the effects of substrate charge and activity upon electroosmotic flow (EOF) in the nanopipette. This allows better informed selection of delivery parameters for the quantitative delivery of neutral species with SICM. Real-time tracking of active dissolution at an enamel surface at the nanoscale is achieved through the controlled delivery of protons and the effects of surface treatments upon the effective heterogeneous rate constant are calculated through FEM. Multiple functional mapping methodologies are combined to probe the bioelectrical properties of live bacteria and FEM is used to further understanding of the structural and dynamic properties of the bacterial cell envelope. Through the combination of experiments with theoretical modelling of the interactions occurring in SICM systems, this work also contributes to understanding of the fundamental processes underpinning SICM measurements.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QC Physics Q Science > QH Natural history T Technology > TA Engineering (General). Civil engineering (General)
Library of Congress Subject Headings (LCSH):	Scanning probe microscopy, Finite element method, Micropipettes
Official Date:	September 2019
Dates:	Date Event September 2019 UNSPECIFIED
Institution:	University of Warwick
Theses Department:	Molecular Analytical Science Centre for Doctoral Training
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Chmel, Nikola Paul ; Unwin, Patrick R. ; Hanna-Brown, Melissa
Format of File:	pdf
Extent:	xiv, 175 leaves : illustrations
Language:	eng

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