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Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay
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Wu, Yun, Wang, Fei, Wang, Chaoyue, Tang, Xinming, Liu, Xianyong, Li, Shaogang, Waterfield, Nicholas R., Wang, Wei, Suo, Xun and Yang, Guowei (2021) Detection of Pneumocystis jirovecii and Toxoplasma gondii in patients with lung infections by a duplex qPCR assay. PLOS Neglected Tropical Diseases, 15 (12). e0010025. doi:10.1371/journal.pntd.0010025 ISSN 1935-2735.
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Official URL: https://doi.org/10.1371/journal.pntd.0010025
Abstract
Pneumocystis pneumonia (PCP) and pulmonary toxoplasmosis (PT) are caused by Pneumocystis jirovecii and Toxoplasma gondii. The clinical symptoms and imaging of PCP and PT are indistinguishable. A duplex qPCR was developed to differentiate between these two pathogens. In testing 92 clinical samples to validate the performance of this method for P. jirovecii detection, it identified 31 positive samples for P. jirovecii infection, consistent with clinical diagnosis. Among the remainder of the 61 clinical samples with suspected PCP, yet showing as negative by the conventional PCR diagnosis approach, 6 of them proved positive using our new assay. Our new approach also produced similar results in identification of T. gondii infections, giving a result of 2 positive and 20 negative in clinical samples. An investigation was undertaken on the prevalence of P. jirovecii and T. gondii infections using 113 samples from lung infection patients. 9% (10/113) were shown to be positive with infections of P. jirovecii, 2% with T. gondii (2/113) and 5% (6/113) were co-infected with both pathogens. Although this duplex qPCR can detect individual P. jirovecii and T. gondii infection, and co-infection of both pathogens, further large-scale investigations are needed to validate its performance, especially in T. gondii detection. Our assay provides a rapid and accurate tool for PCP and PT diagnosis in immunocompromised population and clinical surveillance of these infections in patients with no immune defects.
Item Type: | Journal Article | ||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||||
SWORD Depositor: | Library Publications Router | ||||||
Journal or Publication Title: | PLOS Neglected Tropical Diseases | ||||||
Publisher: | Public Library of Science | ||||||
ISSN: | 1935-2735 | ||||||
Official Date: | 17 December 2021 | ||||||
Dates: |
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Volume: | 15 | ||||||
Number: | 12 | ||||||
Article Number: | e0010025 | ||||||
DOI: | 10.1371/journal.pntd.0010025 | ||||||
Status: | Peer Reviewed | ||||||
Publication Status: | Published | ||||||
Re-use Statement: | ** From PLOS via Jisc Publications Router ** History: received 15-07-2021; accepted 25-11-2021; collection 12-2021; epub 17-12-2021. ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ | ||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||
Date of first compliant deposit: | 9 March 2022 | ||||||
Date of first compliant Open Access: | 9 March 2022 | ||||||
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