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Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice

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Smith, Ciearra B., van der Vinne, Vincent, McCartney, Eleanor, Stowie, Adam C., Leise, Tanya L., Blanca, Martin-Burgos, Molyneux, Penny C., Garbutt, Lauren A, Brodsky, Michael H., Davidson, Alec J., Harrington, Mary E., Dallmann, Robert and Weaver, David R. (2022) Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice. Journal of Biological Rhythms, 37 (1). pp. 53-77. doi:10.11770748/7304211069452

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Official URL: https://doi.org/10.11770748/7304211069452

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Abstract

Circadian rhythms are endogenously generated physiological and molecular rhythms with a cycle length of about 24 h. Bioluminescent reporters have been exceptionally useful for studying circadian rhythms in numerous species. Here, we report development of a reporter mouse generated by modification of a widely expressed and highly rhythmic gene encoding D-site albumin promoter binding protein (Dbp). In this line of mice, firefly luciferase is expressed from the Dbp locus in a Cre recombinase-dependent manner, allowing assessment of bioluminescence rhythms in specific cellular populations. A mouse line in which luciferase expression was Cre-independent was also generated. The Dbp reporter alleles do not alter Dbp gene expression rhythms in liver or circadian locomotor activity rhythms. In vivo and ex vivo studies show the utility of the reporter alleles for monitoring rhythmicity. Our studies reveal cell-type-specific characteristics of rhythms among neuronal populations within the suprachiasmatic nuclei ex vivo. In vivo studies show Dbp-driven bioluminescence rhythms in the liver of Albumin-Cre;DbpKI/+ “liver reporter” mice. After a shift of the lighting schedule, locomotor activity achieved the proper phase relationship with the new lighting cycle more rapidly than hepatic bioluminescence did. As previously shown, restricting food access to the daytime altered the phase of hepatic rhythmicity. Our model allowed assessment of the rate of recovery from misalignment once animals were provided with food ad libitum. These studies confirm the previously demonstrated circadian misalignment following environmental perturbations and reveal the utility of this model for minimally invasive, longitudinal monitoring of rhythmicity from specific mouse tissues.

Item Type: Journal Article
Alternative Title:
Subjects: Q Science > QH Natural history
Q Science > QL Zoology
Q Science > QP Physiology
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): Circadian rhythms, Bioluminescence , Luciferases , Albumins , Cell cycle , Mice as laboratory animals , Biological rhythms -- Mathematical models
Journal or Publication Title: Journal of Biological Rhythms
Publisher: Sage Publications Ltd.
ISSN: 0748-7304
Official Date: 1 February 2022
Dates:
DateEvent
1 February 2022Published
13 January 2022Available
6 December 2021Accepted
Volume: 37
Number: 1
Page Range: pp. 53-77
DOI: 10.11770748/7304211069452
Status: Peer Reviewed
Publication Status: Published
Reuse Statement (publisher, data, author rights): Smith, Ciearra B., van der Vinne, Vincent, McCartney, Eleanor, Stowie, Adam C., Leise, Tanya L., Blanca, Martin-Burgos, Molyneux, Penny C., Garbutt, Lauren A., Brodsky, Michael H., Davidson, Alec J., Harrington, Mary E., Dallmann, Robert and Weaver, David R. (2022) Cell-type specific circadian bioluminescence rhythms in Dbp reporter mice. Journal of Biological Rhythms. 37(1) pp.53-77. Copyright © 2022 (Copyright Holder). Reprinted by permission of SAGE Publications. DOI: https://doi.org/10.11770748/7304211069452 Users who receive access to an article through a repository are reminded that the article is protected by copyright and reuse is restricted to non-commercial and no derivative uses.
Access rights to Published version: Restricted or Subscription Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
R21NS103108National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
SC1GM112567National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
NIGMS R15GM126545National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
UNSPECIFIEDHartmann Müller-Stiftung für Medizinische Forschunghttp://dx.doi.org/10.13039/501100008475
MC_PC_15070 [MRC] Medical Research Councilhttp://dx.doi.org/10.13039/501100000265
R25GM113686National Institutes of Healthhttp://dx.doi.org/10.13039/100000002
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