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Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains

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Gómez-Álvarez, Helena, Iturbe, Pablo, Rivero-Buceta, Virginia, Mines, Paul, Bugg, Tim, Nogales, Juan and Díaz, Eduardo (2022) Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains. Bioresource Technology, 346 . 126638. doi:10.1016/j.biortech.2021.126638

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Official URL: https://doi.org/10.1016/j.biortech.2021.126638

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Abstract

2,4 pyridine dicarboxylic acid (2,4 PDCA) is an analogue of terephthalate, and hence a target chemical in the field of bio-based plastics. Here, Pseudomonas putida KT2440 strains were engineered to efficiently drive the metabolism of lignin-derived monoaromatics towards 2,4 PDCA in a resting cells-based bioprocess that alleviates growth-coupled limitations and allows biocatalysts recycling. Native β-ketoadipate pathway was blocked by replacing protocatechuate 3,4-dioxygenase by the exogenous LigAB extradiol dioxygenase. Overexpression of pcaK encoding a transporter increased 8-fold 2,4 PDCA productivity from protocatechuate, reaching the highest value reported so far (0.58 g L-1 h-1). Overexpression of the 4-hydroxybenzoate monooxygenase (pobA) speed up drastically the production of 2,4 PDCA from 4-hydroxybenzoate (0.056 g L-1 h-1) or p-coumarate (0.012 g L-1 h-1) achieving values 15-fold higher than those reported with Rhodococcus jostii biocatalysts. 2,4 PDCA was also bioproduced by using soda lignin as feedstock, paving the way for future polymeric lignin valorization approaches.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QR Microbiology
T Technology > TP Chemical technology
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Library of Congress Subject Headings (LCSH): Biodegradable plastics, Lignin, Pyridine, Pseudomonas -- Biotechnology
Journal or Publication Title: Bioresource Technology
Publisher: Elsevier BV
ISSN: 0960-8524
Official Date: February 2022
Dates:
DateEvent
February 2022Published
28 December 2021Available
23 December 2021Accepted
Volume: 346
Article Number: 126638
DOI: 10.1016/j.biortech.2021.126638
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
BIO2016-79736-RMinisterio de Ciencia e Innovaciónhttp://dx.doi.org/10.13039/501100004837
PID2019-110612RB-I00Ministerio de Ciencia e Innovaciónhttp://dx.doi.org/10.13039/501100004837
PID2019-108458RB-I00Ministerio de Ciencia e Innovaciónhttp://dx.doi.org/10.13039/501100004837
PCIN-2014-113Ministerio de Ciencia e Innovaciónhttp://dx.doi.org/10.13039/501100004837
PCI2019-111833-2Ministerio de Ciencia e Innovaciónhttp://dx.doi.org/10.13039/501100004837
CSIC 2019 20E005Consejo Superior de Investigaciones Científicashttp://dx.doi.org/10.13039/501100003339
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