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Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains
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Gómez-Álvarez, Helena, Iturbe, Pablo, Rivero-Buceta, Virginia, Mines, Paul, Bugg, Tim, Nogales, Juan and Díaz, Eduardo (2022) Bioconversion of lignin-derived aromatics into the building block pyridine 2,4-dicarboxylic acid by engineering recombinant Pseudomonas putida strains. Bioresource Technology, 346 . 126638. doi:10.1016/j.biortech.2021.126638
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Official URL: https://doi.org/10.1016/j.biortech.2021.126638
Abstract
2,4 pyridine dicarboxylic acid (2,4 PDCA) is an analogue of terephthalate, and hence a target chemical in the field of bio-based plastics. Here, Pseudomonas putida KT2440 strains were engineered to efficiently drive the metabolism of lignin-derived monoaromatics towards 2,4 PDCA in a resting cells-based bioprocess that alleviates growth-coupled limitations and allows biocatalysts recycling. Native β-ketoadipate pathway was blocked by replacing protocatechuate 3,4-dioxygenase by the exogenous LigAB extradiol dioxygenase. Overexpression of pcaK encoding a transporter increased 8-fold 2,4 PDCA productivity from protocatechuate, reaching the highest value reported so far (0.58 g L-1 h-1). Overexpression of the 4-hydroxybenzoate monooxygenase (pobA) speed up drastically the production of 2,4 PDCA from 4-hydroxybenzoate (0.056 g L-1 h-1) or p-coumarate (0.012 g L-1 h-1) achieving values 15-fold higher than those reported with Rhodococcus jostii biocatalysts. 2,4 PDCA was also bioproduced by using soda lignin as feedstock, paving the way for future polymeric lignin valorization approaches.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology T Technology > TP Chemical technology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Biodegradable plastics, Lignin, Pyridine, Pseudomonas -- Biotechnology | |||||||||||||||||||||
| Journal or Publication Title: | Bioresource Technology | |||||||||||||||||||||
| Publisher: | Elsevier BV | |||||||||||||||||||||
| ISSN: | 0960-8524 | |||||||||||||||||||||
| Official Date: | February 2022 | |||||||||||||||||||||
| Dates: |
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| Volume: | 346 | |||||||||||||||||||||
| Article Number: | 126638 | |||||||||||||||||||||
| DOI: | 10.1016/j.biortech.2021.126638 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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