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TheBacillus subtilisgerminant receptor GerA triggers premature germination in response to morphological defects during sporulation
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Ramírez-Guadiana, Fernando H., Meeske, Alexander J., Wang, Xindan, Rodrigues, Christopher D.A. and Rudner, David Z. (2017) TheBacillus subtilisgerminant receptor GerA triggers premature germination in response to morphological defects during sporulation. Molecular Microbiology, 105 (5). pp. 689-704. doi:10.1111/mmi.13728 ISSN 0950-382X.
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Official URL: http://dx.doi.org/10.1111/mmi.13728
Abstract
During sporulation in Bacillus subtilis, germinant receptors assemble in the inner membrane of the developing spore. In response to specific nutrients, these receptors trigger germination and outgrowth. In a transposon-sequencing screen, we serendipitously discovered that loss of function mutations in the gerA receptor partially suppress the phenotypes of > 25 sporulation mutants. Most of these mutants have modest defects in the assembly of the spore protective layers that are exacerbated in the presence of a functional GerA receptor. Several lines of evidence indicate that these mutants inappropriately trigger the activation of GerA during sporulation resulting in premature germination. These findings led us to discover that up to 8% of wild-type sporulating cells trigger premature germination during differentiation in a GerA-dependent manner. This phenomenon was observed in domesticated and undomesticated wild-type strains sporulating in liquid and on solid media. Our data indicate that the GerA receptor is poised on a knife's edge during spore development. We propose that this sensitized state ensures a rapid response to nutrient availability and also elicits premature germination of spores with improperly assembled protective layers resulting in the elimination of even mildly defective individuals from the population.
Item Type: | Journal Article | ||||||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
Journal or Publication Title: | Molecular Microbiology | ||||||||
Publisher: | Wiley | ||||||||
ISSN: | 0950-382X | ||||||||
Official Date: | September 2017 | ||||||||
Dates: |
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Volume: | 105 | ||||||||
Number: | 5 | ||||||||
Page Range: | pp. 689-704 | ||||||||
DOI: | 10.1111/mmi.13728 | ||||||||
Status: | Peer Reviewed | ||||||||
Publication Status: | Published | ||||||||
Access rights to Published version: | Restricted or Subscription Access |
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