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A widely distributed phosphate-insensitive phosphatase presents a route for rapid organophosphorus remineralization in the biosphere
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Lidbury, Ian D. E. A., Scanlan, David J., Murphy, Andrew R. J., Christie-Oleza, Joseph A., Aguilo-Ferretjans, Maria M., Hitchcock, Andrew and Daniell, Tim J. (2022) A widely distributed phosphate-insensitive phosphatase presents a route for rapid organophosphorus remineralization in the biosphere. Proceedings of the National Academy of Sciences, 119 (5). e2118122119. doi:10.1073/pnas.2118122119 ISSN 1091-6490.
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WRAP-widely-distributed-phosphate-insensitive-phosphatase-presents-route-rapid-organophosphorus-remineralization-biosphere-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (3435Kb) | Preview |
Official URL: https://doi.org/10.1073/pnas.2118122119
Abstract
The regeneration of bioavailable phosphate from immobilized organophosphorus represents a key process in the global phosphorus cycle and is facilitated by enzymes known as phosphatases. Most bacteria possess at least one of three phosphatases with broad substrate specificity, known as PhoA, PhoX, and PhoD, whose activity is optimal under alkaline conditions. The production and activity of these phosphatases is repressed by phosphate availability. Therefore, they are only fully functional when bacteria experience phosphorus-limiting growth conditions. Here, we reveal a previously overlooked phosphate-insensitive phosphatase, PafA, prevalent in Bacteroidetes, which is highly abundant in nature and represents a major route for the regeneration of environmental phosphate. Using the enzyme from Flavobacterium johnsoniae, we show that PafA is highly active toward phosphomonoesters, is fully functional in the presence of excess phosphate, and is essential for growth on phosphorylated carbohydrates as a sole carbon source. These distinct properties of PafA may expand the metabolic niche of Bacteroidetes by enabling the utilization of abundant organophosphorus substrates as C and P sources, providing a competitive advantage when inhabiting zones of high microbial activity and nutrient demand. PafA, which is constitutively synthesized by soil and marine flavobacteria, rapidly remineralizes phosphomonoesters releasing bioavailable phosphate that can be acquired by neighboring cells. The pafA gene is highly diverse in plant rhizospheres and is abundant in the global ocean, where it is expressed independently of phosphate availability. PafA therefore represents an important enzyme in the context of global biogeochemical cycling and has potential applications in sustainable agriculture.
| Item Type: | Journal Article | ||||||||
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| Subjects: | Q Science > QD Chemistry Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||||||
| SWORD Depositor: | Library Publications Router | ||||||||
| Library of Congress Subject Headings (LCSH): | Organophosphorus compounds , Organophosphorus compounds -- Environmental aspects , Bacteriology -- Classification, Bacteroides, Phosphatases | ||||||||
| Journal or Publication Title: | Proceedings of the National Academy of Sciences | ||||||||
| Publisher: | National Academy of Sciences | ||||||||
| ISSN: | 1091-6490 | ||||||||
| Official Date: | February 2022 | ||||||||
| Dates: |
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| Volume: | 119 | ||||||||
| Number: | 5 | ||||||||
| Article Number: | e2118122119 | ||||||||
| DOI: | 10.1073/pnas.2118122119 | ||||||||
| Status: | Peer Reviewed | ||||||||
| Publication Status: | Published | ||||||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||||||
| Date of first compliant deposit: | 3 February 2022 | ||||||||
| Date of first compliant Open Access: | 3 February 2022 | ||||||||
| RIOXX Funder/Project Grant: |
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