UNSPECIFIED (1997) Nif gene transfer and expression in chloroplasts: Prospects and problems. In: Meeting on Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes, OCT 13-15, 1996, NATL INST BIOTECHNOL & GENET ENGN, FALSALABAD, PAKISTAN.

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Abstract

The engineering of plants capable of fixing their own nitrogen is an extremely complex task, requiring the coordinated and regulated expression of 16 nif genes in an appropriate cellular location. We suggest that plastids may provide a favourable environment for nif gene expression provided that the nitrogenase enzyme can be protected from oxygen damage. Using the non-heterocystous cyanobacteria as a model, we argue that photosynthesis could be temporally separated from nitrogen fixation in chloroplasts by restricting nitrogenase synthesis to the dark period. We report preliminary data on the introduction and expression of one of nitrogenase components, the Fe protein, in transgenic tobacco and Chlamydomonas reinhardtii. Finally we discuss potential avenues for further research in this area and the prospects for achieving the ultimate goal of expressing active nitrogenase in cereal crops such as rice.

Item Type:	Conference Item (UNSPECIFIED)
Subjects:	S Agriculture S Agriculture > SB Plant culture
Journal or Publication Title:	PLANT AND SOIL
Publisher:	KLUWER ACADEMIC PUBL
ISSN:	0032-079X
Date:	July 1997
Volume:	194
Number:	1-2
Number of Pages:	11
Page Range:	pp. 193-203
Publication Status:	Published
Title of Event:	Meeting on Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes
Location of Event:	NATL INST BIOTECHNOL & GENET ENGN, FALSALABAD, PAKISTAN
Date(s) of Event:	OCT 13-15, 1996
URI:	http://wrap.warwick.ac.uk/id/eprint/16267

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