Nif gene transfer and expression in chloroplasts: Prospects and problems
UNSPECIFIED (1997) Nif gene transfer and expression in chloroplasts: Prospects and problems. In: Meeting on Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes, NATL INST BIOTECHNOL & GENET ENGN, FALSALABAD, PAKISTAN, OCT 13-15, 1996. Published in: PLANT AND SOIL, 194 (1-2). pp. 193-203.Full text not available from this repository.
The engineering of plants capable of fixing their own nitrogen is an extremely complex task, requiring the coordinated and regulated expression of 16 nif genes in an appropriate cellular location. We suggest that plastids may provide a favourable environment for nif gene expression provided that the nitrogenase enzyme can be protected from oxygen damage. Using the non-heterocystous cyanobacteria as a model, we argue that photosynthesis could be temporally separated from nitrogen fixation in chloroplasts by restricting nitrogenase synthesis to the dark period. We report preliminary data on the introduction and expression of one of nitrogenase components, the Fe protein, in transgenic tobacco and Chlamydomonas reinhardtii. Finally we discuss potential avenues for further research in this area and the prospects for achieving the ultimate goal of expressing active nitrogenase in cereal crops such as rice.
|Item Type:||Conference Item (UNSPECIFIED)|
S Agriculture > SB Plant culture
|Journal or Publication Title:||PLANT AND SOIL|
|Publisher:||KLUWER ACADEMIC PUBL|
|Number of Pages:||11|
|Page Range:||pp. 193-203|
|Title of Event:||Meeting on Opportunities for Biological Nitrogen Fixation in Rice and Other Non-Legumes|
|Location of Event:||NATL INST BIOTECHNOL & GENET ENGN, FALSALABAD, PAKISTAN|
|Date(s) of Event:||OCT 13-15, 1996|
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