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Helical ordering of envelope‐associated proteins and glycoproteins in respiratory syncytial virus
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Conley, Michaela J., Short, Judith M., Burns, Andrew M., Streetley, James, Hutchings, Joshua, Bakker, Saskia, Power, B. Joanne, Jaffery, Hussain, Haney, Joanne, Zanetti, Giulia, Murcia, Pablo R., Stewart, Murray, Fearns, Rachel, Vijayakrishnan, Swetha and Bhella, David (2022) Helical ordering of envelope‐associated proteins and glycoproteins in respiratory syncytial virus. The EMBO Journal, 41 (3). e109728. doi:10.15252/embj.2021109728 ISSN 0261-4189.
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Official URL: http://dx.doi.org/10.15252/embj.2021109728
Abstract
Human respiratory syncytial virus (RSV) causes severe respiratory illness in children and the elderly. Here, using cryogenic electron microscopy and tomography combined with computational image analysis and three-dimensional reconstruction, we show that there is extensive helical ordering of the envelope-associated proteins and glycoproteins of RSV filamentous virions. We calculated a 16 Å resolution sub-tomogram average of the matrix protein (M) layer that forms an endoskeleton below the viral envelope. These data define a helical lattice of M-dimers, showing how M is oriented relative to the viral envelope. Glycoproteins that stud the viral envelope were also found to be helically ordered, a property that was coordinated by the M-layer. Furthermore, envelope glycoproteins clustered in pairs, a feature that may have implications for the conformation of fusion (F) glycoprotein epitopes that are the principal target for vaccine and monoclonal antibody development. We also report the presence, in authentic virus infections, of N-RNA rings packaged within RSV virions. These data provide molecular insight into the organisation of the virion and the mechanism of its assembly.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QH Natural history Q Science > QP Physiology Q Science > QR Microbiology R Medicine > RC Internal medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Respiratory syncytial virus, Cryomicroscopy, Electron microscopy, Tomography, Respiratory syncytial virus -- Infections -- Tomography, Glycoproteins , Extracellular matrix proteins, Virus-vector relationships | |||||||||||||||||||||||||||||||||
| Journal or Publication Title: | The EMBO Journal | |||||||||||||||||||||||||||||||||
| Publisher: | Nature Publishing Group | |||||||||||||||||||||||||||||||||
| ISSN: | 0261-4189 | |||||||||||||||||||||||||||||||||
| Official Date: | February 2022 | |||||||||||||||||||||||||||||||||
| Dates: |
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| Volume: | 41 | |||||||||||||||||||||||||||||||||
| Number: | 3 | |||||||||||||||||||||||||||||||||
| Article Number: | e109728 | |||||||||||||||||||||||||||||||||
| DOI: | 10.15252/embj.2021109728 | |||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access (Creative Commons) | |||||||||||||||||||||||||||||||||
| Date of first compliant deposit: | 9 February 2022 | |||||||||||||||||||||||||||||||||
| Date of first compliant Open Access: | 10 February 2022 | |||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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