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An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale
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de Figueiredo, Marcelo R. A., Küpper, Anita, Malone, Jenna M., Petrovic, Tijana, de Figueiredo, Ana Beatriz T. B., Campagnola, Grace, Peersen, Olve B., Prasad, Kasavajhala V.S.K., Patterson, Eric L., Reddy, Anireddy S.N., Kubeš, Martin F., Napier, Richard, Preston, Christopher and Gaines, Todd A. (2022) An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale. Proceedings of the National Academy of Sciences of the United States of America, 119 (9). e2105819119. doi:10.1073/pnas.2105819119
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Official URL: https://doi.org/10.1073/pnas.2105819119
Abstract
The natural auxin indole-3-acetic acid (IAA) is a key regulator of many aspects of plant growth and development. Synthetic auxin herbicides such as 2,4-D mimic the effects of IAA by inducing strong auxinic-signaling responses in plants. To determine the mechanism of 2,4-D resistance in a Sisymbrium orientale (Indian hedge mustard) weed population, we performed a transcriptome analysis of 2,4-D-resistant (R) and -susceptible (S) genotypes that revealed an in-frame 27-nucleotide deletion removing nine amino acids in the degron tail (DT) of the auxin coreceptor Aux/IAA2 (SoIAA2). The deletion allele cosegregated with 2,4-D resistance in recombinant inbred lines. Further, this deletion was also detected in several 2,4-D-resistant field populations of this species. Arabidopsis transgenic lines expressing the SoIAA2 mutant allele were resistant to 2,4-D and dicamba. The IAA2-DT deletion reduced binding to TIR1 in vitro with both natural and synthetic auxins, causing reduced association and increased dissociation rates. This mechanism of synthetic auxin herbicide resistance assigns an in planta function to the DT region of this Aux/IAA coreceptor for its role in synthetic auxin binding kinetics and reveals a potential biotechnological approach to produce synthetic auxin-resistant crops using gene-editing.
| Item Type: | Journal Article | |||||||||||||||||||||
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| Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | |||||||||||||||||||||
| Library of Congress Subject Headings (LCSH): | Dicamba, Herbicide resistance, Plant hormones -- Synthesis, Cruciferae | |||||||||||||||||||||
| Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | |||||||||||||||||||||
| Publisher: | National Academy of Sciences | |||||||||||||||||||||
| ISSN: | 0027-8424 | |||||||||||||||||||||
| Book Title: | An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale | |||||||||||||||||||||
| Official Date: | 1 March 2022 | |||||||||||||||||||||
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| Volume: | 119 | |||||||||||||||||||||
| Number: | 9 | |||||||||||||||||||||
| Article Number: | e2105819119 | |||||||||||||||||||||
| DOI: | 10.1073/pnas.2105819119 | |||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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