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An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale

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de Figueiredo, Marcelo R. A., Küpper, Anita, Malone, Jenna M., Petrovic, Tijana, de Figueiredo, Ana Beatriz T. B., Campagnola, Grace, Peersen, Olve B., Prasad, Kasavajhala V.S.K., Patterson, Eric L., Reddy, Anireddy S.N., Kubeš, Martin F., Napier, Richard, Preston, Christopher and Gaines, Todd A. (2022) An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale. Proceedings of the National Academy of Sciences of the United States of America, 119 (9). e2105819119. doi:10.1073/pnas.2105819119 ISSN 0027-8424.

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Official URL: https://doi.org/10.1073/pnas.2105819119

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Abstract

The natural auxin indole-3-acetic acid (IAA) is a key regulator of many aspects of plant growth and development. Synthetic auxin herbicides such as 2,4-D mimic the effects of IAA by inducing strong auxinic-signaling responses in plants. To determine the mechanism of 2,4-D resistance in a Sisymbrium orientale (Indian hedge mustard) weed population, we performed a transcriptome analysis of 2,4-D-resistant (R) and -susceptible (S) genotypes that revealed an in-frame 27-nucleotide deletion removing nine amino acids in the degron tail (DT) of the auxin coreceptor Aux/IAA2 (SoIAA2). The deletion allele cosegregated with 2,4-D resistance in recombinant inbred lines. Further, this deletion was also detected in several 2,4-D-resistant field populations of this species. Arabidopsis transgenic lines expressing the SoIAA2 mutant allele were resistant to 2,4-D and dicamba. The IAA2-DT deletion reduced binding to TIR1 in vitro with both natural and synthetic auxins, causing reduced association and increased dissociation rates. This mechanism of synthetic auxin herbicide resistance assigns an in planta function to the DT region of this Aux/IAA coreceptor for its role in synthetic auxin binding kinetics and reveals a potential biotechnological approach to produce synthetic auxin-resistant crops using gene-editing.

Item Type: Journal Article
Subjects: Q Science > QK Botany
S Agriculture > SB Plant culture
Divisions: Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- )
Library of Congress Subject Headings (LCSH): Dicamba, Herbicide resistance, Plant hormones -- Synthesis, Cruciferae
Journal or Publication Title: Proceedings of the National Academy of Sciences of the United States of America
Publisher: National Academy of Sciences
ISSN: 0027-8424
Book Title: An in-frame deletion mutation in the degron tail of auxin co-receptor IAA2 confers resistance to the herbicide 2,4-D in Sisymbrium orientale
Official Date: 1 March 2022
Dates:
DateEvent
1 March 2022Published
9 January 2021Accepted
Volume: 119
Number: 9
Article Number: e2105819119
DOI: 10.1073/pnas.2105819119
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Date of first compliant deposit: 17 February 2022
Date of first compliant Open Access: 28 February 2022
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
UNSPECIFIEDDow AgroSciences LLChttp://viaf.org/viaf/1072152200774614400009
UA00158Grains Research and Development Corporationhttp://dx.doi.org/10.13039/501100000980
UNSPECIFIEDNational Council for Scientific and Technological DevelopmentUNSPECIFIED
207387/2014-1Conselho Nacional de Desenvolvimento Científico e Tecnológicohttp://dx.doi.org/10.13039/501100003593
792329H2020 Marie Skłodowska-Curie Actionshttp://dx.doi.org/10.13039/100010665
BB/M017982/1, WISB centerUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
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