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Plasmonic detection of SARS-CoV-2 spike protein with polymer-stabilized glycosylated gold nanorods
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Georgiou, Panagiotis G., Guy, Collette S., Hasan, Muhammad, Ahmad, Ashfaq, Richards, Sarah-Jane, Baker, Alexander N., Thakkar, Neer V., Walker, Marc, Pandey, Sarojini, Anderson, Neil R., Grammatopoulos, Dimitris and Gibson, Matthew I. (2022) Plasmonic detection of SARS-CoV-2 spike protein with polymer-stabilized glycosylated gold nanorods. ACS Macro Letters, 11 . pp. 317-322. doi:10.1021/acsmacrolett.1c00716
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WRAP-Plasmonic-detection-SARS-CoV‑2-spike-protein-polymer-glycosylated-gold-nanorods-2022.pdf - Published Version - Requires a PDF viewer. Available under License Creative Commons Attribution 4.0. Download (2581Kb) | Preview |
Official URL: https://doi.org/10.1021/acsmacrolett.1c00716
Abstract
The COVID-19 pandemic has highlighted the need for innovative biosensing, diagnostic, and surveillance platforms. Here we report that glycosylated, polymer-stabilized, gold nanorods can bind the SARS-CoV-2 spike protein and show correlation to the presence of SARS-CoV-2 in primary COVID-19 clinical samples. Telechelic polymers were prepared by reversible addition–fragmentation chain-transfer polymerization, enabling the capture of 2,3-sialyllactose and immobilization onto gold nanorods. Control experiments with a panel of lectins and a galactosamine-terminated polymer confirmed the selective binding. The glycosylated rods were shown to give dose-dependent responses against recombinant truncated SARS-CoV-2 spike protein, and the responses were further correlated using primary patient swab samples. The essentiality of the anisotropic particles for reducing the background interference is demonstrated. This highlights the utility of polymer tethering of glycans for plasmonic biosensors of infection.
| Item Type: | Journal Article | |||||||||||||||||||||||||||||||||
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| Subjects: | Q Science > QC Physics Q Science > QR Microbiology > QR355 Virology R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry Faculty of Science, Engineering and Medicine > Science > Physics Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | COVID-19 (Disease) -- Diagnosis, Metal nanoparticles, Biosensors, Plasmonics | |||||||||||||||||||||||||||||||||
| Journal or Publication Title: | ACS Macro Letters | |||||||||||||||||||||||||||||||||
| Publisher: | American Chemical Society | |||||||||||||||||||||||||||||||||
| ISSN: | 2161-1653 | |||||||||||||||||||||||||||||||||
| Official Date: | 20 February 2022 | |||||||||||||||||||||||||||||||||
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| Volume: | 11 | |||||||||||||||||||||||||||||||||
| Page Range: | pp. 317-322 | |||||||||||||||||||||||||||||||||
| DOI: | 10.1021/acsmacrolett.1c00716 | |||||||||||||||||||||||||||||||||
| Status: | Peer Reviewed | |||||||||||||||||||||||||||||||||
| Publication Status: | Published | |||||||||||||||||||||||||||||||||
| Access rights to Published version: | Open Access | |||||||||||||||||||||||||||||||||
| Copyright Holders: | © 2022 The Authors. Published by American Chemical Society | |||||||||||||||||||||||||||||||||
| RIOXX Funder/Project Grant: |
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