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Plasmonic detection of SARS-CoV-2 spike protein with polymer-stabilized glycosylated gold nanorods

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Georgiou, Panagiotis G., Guy, Collette S., Hasan, Muhammad, Ahmad, Ashfaq, Richards, Sarah-Jane, Baker, Alexander N., Thakkar, Neer V., Walker, Marc, Pandey, Sarojini, Anderson, Neil R., Grammatopoulos, Dimitris and Gibson, Matthew I. (2022) Plasmonic detection of SARS-CoV-2 spike protein with polymer-stabilized glycosylated gold nanorods. ACS Macro Letters, 11 . pp. 317-322. doi:10.1021/acsmacrolett.1c00716 ISSN 2161-1653.

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Official URL: https://doi.org/10.1021/acsmacrolett.1c00716

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Abstract

The COVID-19 pandemic has highlighted the need for innovative biosensing, diagnostic, and surveillance platforms. Here we report that glycosylated, polymer-stabilized, gold nanorods can bind the SARS-CoV-2 spike protein and show correlation to the presence of SARS-CoV-2 in primary COVID-19 clinical samples. Telechelic polymers were prepared by reversible addition–fragmentation chain-transfer polymerization, enabling the capture of 2,3-sialyllactose and immobilization onto gold nanorods. Control experiments with a panel of lectins and a galactosamine-terminated polymer confirmed the selective binding. The glycosylated rods were shown to give dose-dependent responses against recombinant truncated SARS-CoV-2 spike protein, and the responses were further correlated using primary patient swab samples. The essentiality of the anisotropic particles for reducing the background interference is demonstrated. This highlights the utility of polymer tethering of glycans for plasmonic biosensors of infection.

Item Type: Journal Article
Subjects: Q Science > QC Physics
Q Science > QR Microbiology > QR355 Virology
R Medicine > R Medicine (General)
R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Divisions: Faculty of Science, Engineering and Medicine > Science > Chemistry
Faculty of Science, Engineering and Medicine > Science > Physics
Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH): COVID-19 (Disease) -- Diagnosis, Metal nanoparticles, Biosensors, Plasmonics
Journal or Publication Title: ACS Macro Letters
Publisher: American Chemical Society
ISSN: 2161-1653
Official Date: 20 February 2022
Dates:
DateEvent
20 February 2022Published
20 January 2022Accepted
Volume: 11
Page Range: pp. 317-322
DOI: 10.1021/acsmacrolett.1c00716
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Open Access (Creative Commons)
Copyright Holders: © 2022 The Authors. Published by American Chemical Society
Date of first compliant deposit: 21 February 2022
Date of first compliant Open Access: 23 February 2022
RIOXX Funder/Project Grant:
Project/Grant IDRIOXX Funder NameFunder ID
814236[ERC] Horizon 2020 Framework Programmehttp://dx.doi.org/10.13039/100010661
BB/M01116X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
StudentshipIceni Diagnosticshttps://www.iceniglycoscience.com/
BB/M02878X/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268
866056European Research Councilhttp://dx.doi.org/10.13039/501100000781
Industry Fellowship 191037Royal Societyhttp://dx.doi.org/10.13039/501100000288
RPG-2019-087Leverhulme Trusthttp://dx.doi.org/10.13039/501100000275
UNSPECIFIEDUniversity of Warwickhttp://dx.doi.org/10.13039/501100000741
EP/R511808/1[EPSRC] Engineering and Physical Sciences Research Councilhttp://dx.doi.org/10.13039/501100000266
BB/S506783/1[BBSRC] Biotechnology and Biological Sciences Research Councilhttp://dx.doi.org/10.13039/501100000268

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